Test Information

To locate information on a particular test, click on the relevant letter and follow the trail. eg To find information on sample requirements, test availability and turnaround time for FeLV, click on "F" and then click on the relevant test (FeLV soluble Ag or FeLV IFA).

To download a copy of our lab request form please click link below:

LAB REQUEST FORM


CAE (Caprine arthritis encephalitis)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 4 mL whole blood

 

Turnaround Time:
  • 10 working days

 

Request/Test Included in:
  • Request Test

 

Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes: N/A
Internal Laboratory: Dept of Ag WA
Internal Test Volume: Serum from 4 mL clotted blood
Calcium (Ca)
Suitable Sample:
  • PC
  • LH
Unsuitable Sample:
  • EDTA
  • FL
  • CIT 
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Request test
  • CP 2, 6
  • FP 2, 5
  • EP 3
  • RP 1, 2, 3, 4
  • AP 1, 2, 3, 4
Comments:
  • May be affected by haemolysis and/or lipaemia.

 

Internal Use Only:
  • Always run concurrent albumin
  • See Interferogram; lipemia increases, hemolysis decreases
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 25 uL (Ca only)
Calcium (Ca) - Ionised
Suitable Sample:
  • PC (See the comments below regarding the age of suitable samples)
Unsuitable Sample:
  • LH
  • EDTA
  • CIT
Minimum Sample Size:
  • Minimum 1ml whole blood ensuring that the tube is filled to the nominated capacity.

 

Turnaround Time:
  • 1 day; Monday - Friday

 

Request/Test Included in:
  • Request test, please contact Vetpath before submitting sample for fee (dependent on taxi fare).

 

Comments:
  • Sample must be as fresh as possible; preferably less than 2 hours but up to 4 hours is OK.
  • The result is affected by pH and storage.
  • Treat as a sample collected for blood gas analysis. 
  • Once collected DO NOT REMOVE LID.
  • If you are requesting other tests which use clotted blood in addition to ionised calcium please include a separate sample for those tests.
Internal Use Only:
  • Samples can be taxied straight from the vet clinic to WDP.
  • Spin to separate DO NOT REMOVE LID, store cold not frozen. 
  • Send directly to WDP Myaree (via taxi if necessary). If Myaree unavail then send to Joondalup
  • Suite 3 Specialist Medical Centre East Cnr Shenton Ave and Grand Boulevard Joondalup ph:9400 9810.


Internal Codes: B40
Internal Laboratory: WDP Myaree
Internal Test Volume:
Calicivirus - Rabbit
Suitable Sample:
  • Liver (fresh or frozen)

 

Unsuitable Sample:
  • Blood

 

Minimum Sample Size:
  • Amount not stated

 

Turnaround Time:
  • Run once weekly, allow 5-10 working days

 

Request/Test Included in:
  • Request test

 

Comments:
  • Test detects antigen
Internal Use Only: {Notes for Internal Use}
Internal Codes: N/A
Internal Laboratory: Dep't of Ag, WA
Internal Test Volume: N/A
Calicivirus (feline) Antibody Titre by IFA
(Feline Calicivirus Antibody Titre by IFA)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run Tuesday and Thursday
  • Cut off is 7pm the previous working day
  • Results available the same day
Request/Test Included in:
  • Individual test
  • Combined feline calicivirus, herpes virus & panleukopenia
Comments: This test is a screening test to determine the level of specific antiviral IgG in the serum. The titre is used as a measure of the humoral immunity. It has been shown that a titre of 1:5 on IFA is considered the appropriate levels to provide protective immunity. Note that it is possible that lower titres are protective and the influence of the cell mediated response cannot be reliably assessed in this manner.

  • Cats with a titre of <1:5 may not be adequately protected and should be revaccinated.
  • Cats with a titre of 1:5 but <1:20 should consider revaccination.
  • Cats with a titre of 1:20 or greater are considered protected and may not need revaccination. These animals should have their titre rechecked in approximately one year.
Internal Use Only:
  • Store sample at 2-8oC
  • Serial dilutions are preformed from 1:5 with four-fold up to 1:80 (greater dilutions may be requested in some cases, charges are negotiable).
  • If the test is being done to detect exposure to calicivirus, acute specimens should be drawn at the start of illness and convalescent specimens should be obtained at 2 to 4 week intervals to check titre changes.
  • Samples can still be sent to Biobest in Scotland if the client requests this.
Internal Codes:
  • S26F
  • S26L (for laboratories)
  • S24F (combination of calici, herpes & panleukpenia)
  • S24L (combination of calici, herpes & panleukpenia for laboratories)
Internal Laboratory: VP
Internal Test Volume:
  • 50-100 uL serum for the individual test
  • 100-200 uL is needed for the combination
Calicivirus (feline) Antibody Titre by VN
(Feline Calicivirus Antibody Titre by VN)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
  • FL
Minimum Sample Size:
  • 3 ml whole blood

 

Turnaround Time:
  • Approximately 2 weeks

 

Request/Test Included in:
  • Request Test (must specifically request antibody titre by virus neutralisation test/VNT)
  • Contact laboratory if doing multiple tests for a client
Comments:
  • Virus neutralisation test (VNT)

 

Internal Use Only:
  • Samples are sent to Scotland on Mondays and results are returned within two weeks.

 

Internal Codes:
  • S26B
  • S26B+ (additional tests in same batch for same client)
Internal Laboratory: Biobest, Scotland
Internal Test Volume: 1 ml clear serum
Calicivirus by PCR
(Feline Respiratory Profile by PCR - Calicivirus, Herpes & Chlamydia)
Suitable Sample:
  • Swab of tears or exudate (see Comments)

 

Unsuitable Sample:
  • Sample in transport medium.
  • Frozen sample.
Minimum Sample Size:
  • Single swab

 

Turnaround Time:
  • 5-10 days depending on day sample received.
  • Contact the laboratory to confirm.
Request/Test Included in: Feline respiratory profile by PCR (not available as a single test)
Comments:
  • Use a dry sterile cotton swab to collect the sample.
  • Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs or the appropriate lesion.
  • Place the swab in a sterile container and refrigerate.
  • Do not freeze.
  • Do not place in transport medium.
Internal Use Only:
  • Referral lab- WDP Myaree Molecular Department

  • Tested as required (results usually issued within 5 working weekdays from time of referral to WDP)

 

Internal Codes: G11
Internal Laboratory: WDP
Internal Test Volume:
Campylobacter Culture
Suitable Sample:
  • Faeces

 

Unsuitable Sample:
  • Blood

  • Urine

Minimum Sample Size:
Turnaround Time: Allow up to 5 working days, but check with the laboratory
Request/Test Included in:
  • Request Campylobacter culture

 

Comments:
  • Campylobacter does not grow under routine conditions used for faecal culture

 

Internal Use Only: Handle with care as Campylobacter is a zoonosis.
Internal Codes: F12
Internal Laboratory: WDP .... need to check on any specific requirements
Internal Test Volume:
Canine Adenovirus Antibody Titre (Infectious Canine Hepatitis)
(Adenovirus Antibody Titre)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
  • FL
Minimum Sample Size:
  • 3 ml whole blood

 

Turnaround Time:
  • Up to 4 weeks

 

Request/Test Included in:
  • Request Test

 

Comments:
  • Samples are sent on Mondays and results are returned within two weeks.
  • Can be done as an individual test, or as a combination (distemper, parvovirus, adenovirus).

 

Internal Use Only:
  • Canine adenovirus (infectious canine hepatitis) testing is done at Biobest in Scotland
  • Unless specifically requested, distemper and parvovirus antibody titres requested at the same time as an adenovirus titre are done at Vetpath and only the adenovirus test sent to Biobest.
Internal Codes:
  • S13B (adenovirus by VN)
  • S15B (combination distemper, parvovirus and adenovirus ONLY IF ALL are going to Biobest) 
  • U
Internal Laboratory: Biobest, Scotland
Internal Test Volume: 1 ml clear serum
Canine Distemper Virus Antibody Titre by IFA
(Distemper Virus Antibody Titre by IFA)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • CDV IFA titre is run Tuesday & Thursday
  • Cutoff time 7pm previous working weekday
  • Results available the same day
Request/Test Included in:
  • CDV antibody titres by IFA 
  • Combined CDV & CPV IFA titre (sample requirements are the same as for CDV alone, both tests run the same day)
Comments: Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations.  Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.

Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not.  This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests. Further studies have shown that the IFA method is comparable to the gold standard haemagglutination test for CPV and the virus neutralisation test for CDV.

INTERPRETATION:
  • Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
  • Titres of >1:5 are considered seropositive.
  • Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
  • Dogs with titres of >1:20 probably do not require revaccination.
Internal Use Only:
  • Original file 21-07-2004
  • Serial dilutions are performed from 1:5 with four fold diltions to 1:80 (greater dilutions may be requested in some instances - charges are negotiable.
  • Samples can still be sent to Biobest in Scotland; this is done only if the client specifically requests it.
  • Store samples at 2-8oC
Internal Codes:
  • S12F (CDV  by IFA)
  • S14F (combination CDV & CPV by IFA, no adenovirus)
  • S12L (CDV by IFA for laboratory)
  • S14L (combination CDV and CPV for laboratory)
Internal Laboratory: VP
Internal Test Volume: 100 uL to 200 uL is needed per test 
Canine Distemper Virus Antibody Titre by VN
(Distemper Virus Antibody Titre by VN)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
  • FL
Minimum Sample Size:
  • 3 ml whole blood

 

Turnaround Time:
  • up to 4 weeks

 

Request/Test Included in:
  • Request test

 

Comments:
  • Distemper serology can also be done by IFA at Vetpath
Internal Use Only:
  • If only canine distemper serology is required, use IFA
  • The VN (virus neutralisation) test is used only when specifically requested by the client
  • Samples are sent on Mondays and results are returned within two weeks.
Internal Codes:
  • S12B Distemper Ab by VN (virus neutralisation)
  • S15B Combination distemper, parvo and adenovirus all done at Biobest 
Internal Laboratory: Biobest, Scotland
Internal Test Volume: 1 ml clear serum
Canine Leptospira PCR
(Leptospira PCR)
Suitable Sample:
  • Urine
  • EDTA blood
Unsuitable Sample:
  • PC
Minimum Sample Size:
  • 1ml
Turnaround Time:
  • 5 - 7 days
Request/Test Included in:

Request test

Comments:
  • Detects Leptospira interrogans by PCR
Internal Use Only:
Internal Codes: G37
Internal Laboratory: Vetnostics
Internal Test Volume:
Canine neurological PCR panel
(Neurological PCR panel - canine)
Suitable Sample:
  • CSF
Unsuitable Sample:
  • Blood
Minimum Sample Size:
  • 0.5ml
Turnaround Time:
  • 3 working days
Request/Test Included in:
  • Request test
Comments:

Panel includes:

  • Toxoplama gondii
  • Cryptococcus sp.
  • Angiostrongylus sp
  • Neospora caninum
  • Canine Distemper virus
Internal Use Only:
Internal Codes: G34
Internal Laboratory: Vetnostics
Internal Test Volume:
Canine Parvovirus Antibody Titre by HAI (Biobest)
(Parvovirus (canine) titre by HAI at Biobest)
Suitable Sample:
  • PC
Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 3ml whole blood
Turnaround Time:
  • Up to 4 weeks
Request/Test Included in:
  • Request test (must specify Biobest)
Comments:
    • Performed by haemagglutination inhibition
Internal Use Only:
Internal Codes: S11B
Internal Laboratory: Biobest
Internal Test Volume:
Canine Parvovirus Antigen (Faeces)
(Parvovirus Antigen (Faeces))
Suitable Sample:
  • Faeces
  • Faecal swab
Unsuitable Sample:
  • Blood

 

Minimum Sample Size:
  • Faecal swab

 

Turnaround Time:
  • 2 hours

 

Request/Test Included in:
  • Request test

 

Comments:
  • False negative results may occur as the period of viral shedding in the faeces is relatively brief and virus is seldom detected 10 to 12 days after infection (this corresponds to 5 to 7 days of clinical illness).
  • False negatives may also occur very early in infection; retest in 36 to 48 hours if this is suspected.
  • Modified live vaccination results in transient faecal shedding and may give a weak positive result 5-15 days after vaccination.
Internal Use Only: AGEN Witness Parvo test in use
Internal Codes: S11A
Internal Laboratory: VP
Internal Test Volume:
Canine Parvovirus Antibody Titre by IFA
(Parvovirus Antibody Titre by IFA)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run Tuesday & Thursday (both CDV & CPV)
  • Cutoff time 7pm previous working weekday
  • Results same day
Request/Test Included in:
  • CPV antibody titre by IFA 
  • Combined CDV & CPV IFA titres
Comments:
Biobest now perform CPV titre testing by IFA (no longer HAI).

Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations.  Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.


Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not.  This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests. Further studies have shown that the IFA method is comparable to the virus neutralisation test for CDV.

INTERPRETATION:
  • Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
  • Titres of >1:5 are considered seropositive.
  • Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
  • Dogs with titres of >1:20 probably do not require revaccination.
Internal Use Only:
  • Store at 2-8oC
  • Original file 21-07-2004
  • Serial dilutions are performed from 1:5 with four fold diltions to 1:8- (greater dilutions may be requested in some instances - charges are negotiable).
  • The use of EDTA or LH plasma has not been evaluated.
  • Samples can still be sent to Biobest in Scotland; this is done only if the client specifically requests it.

 

Internal Codes:
  • S11F (CPV by IFA)
  • S11L (CPV by IFA for a laboratory)
  • S14F (combinationCPV & CDV by IFA)
  • S14L (combinationCPV & CDV by IFA for laboratoried)
Internal Laboratory: VP
Internal Test Volume:
  • 50 uL for CPV IFA titre
  • 100-200 uL for both CPV and CDV
Canine Respiratory panel by PCR
(Respiratory PCR panel - Canine)
Suitable Sample:
  • Nasal or deep pharyngeal swab (not in culture media)
  • BAL or TTA
Unsuitable Sample:
  • Blood
Minimum Sample Size:
  • Swab or 1ml fluid
Turnaround Time:
  • 7 working days
Request/Test Included in:
  • Request test
Comments:

Panel includes:

  • Bordetella bronchiseptica
  • Streptococcus equi subsp zooepidemicus
  • Canine Adenovirus type 2
  • Canine Herpesvirus type 1
  • Coronavirus
  • Canine Parainfluenza virus
  • Mycoplasma cynos
Internal Use Only:
Internal Codes: G33
Internal Laboratory: Vetnostics
Internal Test Volume:
Canine Seizure Panel
(Seizure Panel - canine)
Suitable Sample:
  • EDTA (hematology component of CP2) 
    PLUS
  • PC (biochemisty & serology)
    PLUS 
  • FL (glucose)
    PLUS
  • Urine for the SG component of the CP2
Unsuitable Sample:
  • LH
  • SC

 

Minimum Sample Size:
  • 2 mL of whole blood
Turnaround Time:
  • CP2: Same day (Mon-Sat)
  • Cryptococcus Titre: Same day (Mon-Friday 3:00 pm cutoff, Saturday 1:00 pm cutoff)
  • Neospora & Toxoplasma serology: Run Tuesday & Friday with a 10 am cutoff.  Results for these tests are usually available later that day.
Request/Test Included in:
  • Canine Seizure Panel (CSP)
Comments: This panel includes: 
  • CP2
  • Neospora IgG IFA
  • Toxoplasma IgG & IgM IFA
  • Cryptococcus titre
Internal Use Only:
Internal Codes: CSP
Internal Laboratory: VP
Internal Test Volume:
Canine Trypsin-like Immunoreactivity (cTLI)
(Trypsin-like Immunoreactivity (cTLI) - canine)
Suitable Sample:
  • PC (canine only)
  • An overnight (>6 hours) fast is recommended prior to blood collection
Unsuitable Sample:
  • EDTA
  • LH
  • FL
  • CIT
  • Species other than canine
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run Monday to Friday 10:30am cut off
  • Results available the same day
Request/Test Included in:
  • Request test

 

Comments:
  • Sensitive and specific test for the diagnosis of canine exocrine pancreatic insufficiency (EPI)
  • Reference range for healthy dogs is 5.4 - 32 ng/mL
  • Values <2.5 ng/mL are diagnostic for canine exocrine pancreatic insufficiency.
  • Dogs with values 2.5 - 5.0 ng/mL should be retested in one month.
Internal Use Only:
  • Run on Immulite
  • Back-up is Melbourne Uni
Internal Codes: E30
Internal Laboratory: VP
Internal Test Volume: Needs a minimum of 150 uL of serum
Cannabis (THC, Tetrahydrocannabinol, Marijuana)
Suitable Sample:
  • Urine

 

Unsuitable Sample:
  • Blood

 

Minimum Sample Size:
  • 0.5 mL

 

Turnaround Time:
  • 2 working days

 

Request/Test Included in:
  • Request test
  • Recreational drug screen
Comments:
  • Can be detected in urine for up to 10 days post-ingestion
  • Can also be done as part of the recreational drug screen
Internal Use Only: {Notes for Internal Use}
Internal Codes: X10
Internal Laboratory: Clinipath
Internal Test Volume: N/A
Cardiac troponin I
(Tropinin I)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • SC
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 2-3 working days

 

Request/Test Included in:
  • Special request only - please contact the laboratory

 

Comments:
  • Troponin I in serum increases in response to myocardial injury.
  • Not routine test - special request only (trial return).
Internal Use Only: As per DB - 25-11-2008? - Samples to be sent to WDP

As per DB - 25/11 - Samples to be sent to WDP.

[Were previously sent to PathLab, attention Ron Bell, 52 Childs Road, Epping, Victoria 3076 ph 03 9408 9817 fax 03 9401 5822]
Internal Codes: E21
Internal Laboratory: WDP
Internal Test Volume: Minimum 150 uL? Not sure on this, from previous information.
CBC (Complete Blood Count)
Suitable Sample:
  • EDTA
  • CIT if less than 1 day old
  • LH for some birds and reptiles
Unsuitable Sample:
  • PC
  • FL
Minimum Sample Size:
  • 1.0 mL, but prefer 2 mL

 

Turnaround Time:
  • < 3 hours, less if marked URGENT

 

Request/Test Included in:
  • CBC
  • Full coagulation screen
  • CP1, 2, 3, 4
  • FP1, 2, 3
  • EP1, 2, 3, 4, 5
  • RP1, 2
  • AP1, 2
Comments:
  • LH can be used for a CBC, but there is significant clumping of platelets and WBC (affecting the total and differential counts), and the fibrinogen cannot be done.
Internal Use Only: {Notes for Internal Use}
Internal Codes:
  • H01
  • H02 repeat/add-on
  • H03 part
    Internal Laboratory: VP
    Internal Test Volume: N/A
    CEM (Contagious Equine Metritis, Taylorella)
    Suitable Sample:
    • Aimes medium with charcoal swabs (see comments)

     

    Unsuitable Sample:
    • Normal transport media swabs

     

    Minimum Sample Size:
    • Mares: 1 swab
    • Stallions: 3 swabs
    Turnaround Time:
    • At least 48 hours

     

    Request/Test Included in:
    • Request CEM culture; please phone laboratory in advance.

     

    Comments:
    • Need to use Aimes with charcoal swabs.

     

    Internal Use Only: {Notes for Internal Use}
    Internal Codes:
    Internal Laboratory: Department of Agriculture WA
    Internal Test Volume: N/A
    Chlamydia - Feline by PCR
    (Feline Respiratory Profile by PCR - Calicivirus, Herpes & Chlamydia)
    Suitable Sample:
    • Swab of tears or exudate (see Comments)

     

    Unsuitable Sample:
    • Sample in transport medium.
    • Frozen sample.
    Minimum Sample Size:
    • Single swab

     

    Turnaround Time:
    • 5-10 days depending on day sample received.
    • Contact the laboratory to confirm.
    Request/Test Included in:
    • Feline respiratory profile by PCR (not available as a single test)
    Comments:
    • Use a dry sterile cotton swab to collect the sample.
    • Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs or the appropriate lesion.
    • Place the swab in a sterile container and refrigerate.
    • Do not freeze.
    • Do not place in transport medium.
    Internal Use Only:

     

    • Referral lab- WDP Myaree Molecular Department

    • Tested as required (results usually issued within 5 working weekdays from time of referral to WDP)
    • Chylamdia for species other than feline refer to Melb Uni

       

    Internal Codes: G11
    Internal Laboratory: WDP
    Internal Test Volume:
    Chlamydia - IFA (Feline & Avian)
    Suitable Sample:
    • 2 air-dried smears of exudate (area the size of a 5 cent coin on each slide)
    • Feline samples: conjunctival or exudate
    • Avian samples: cloacal swab/smear, fresh liver
    • Do NOT refrigerate
    Unsuitable Sample:
    • Samples in transport media.

     

    Minimum Sample Size:
    • 1 smear

     

    Turnaround Time:
    • Monday - Friday on demand
    • 3:00 pm cutoff
    • Results by 6 pm
    Request/Test Included in:
    • Request test

     

    Comments:
    •  Do not refrigerate smears.

    •  

    Internal Use Only: {Notes for Internal Use}
    Internal Codes:
    • P02
    • P03
    Internal Laboratory: VP
    Internal Test Volume: N/A
    Chlamydia - Koala by PCR
    (Koala - Chlamydia by PCR)
    Suitable Sample:
    • Conjunctival swab
      AND/OR
    • Cloacal swab
    • See below for sample collection
    Unsuitable Sample:
    • Blood
    • Swabs in transport medium
    Minimum Sample Size:
    • One swab
    • If more than one swab is submitted per animal, each swab is charged for separately
    Turnaround Time:
    • 5-10 days depending on the day the sample is received
    • Contact the laboratory to confirm expected turnaround time
    Request/Test Included in:
    • Chlamydia PCR for koala

     

    Comments:
    • Use a dry sterile cotton swab to collect the sample.
    • Moisten the swab with tears or exudate and vigorously swab both conjunctival sacs and/or cloaca.
    • Place the swab in a sterile container and refrigerate.
    • Use a separate container for each animal if collecting from more than one animal.
    • Do not freeze.
    • Do not place in transport medium.
    Internal Use Only:
    • See Client Information Sheet DCI-10 (this is for the Feline respiratory panel, but gives the collection method)
    • Run each Tuesday morning, results available Tuesday afternoon.
    • Samples must arrive at Vetpath by 11 am Thursday before the test is run.
    Internal Codes:
    • G03
    Internal Laboratory:
    • University of Melbourne PCR Laboratory
    Internal Test Volume: N/A
    Chloride
    Suitable Sample:
    • PC
    • LH
    Unsuitable Sample:
    • EDTA
    • FL
    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 4 hours

     

    Request/Test Included in:
    • Request test
    • CP2, 6
    • FP2, 5
    • EP2, 3, 5, 7
    • RP2, 4
    • AP2, 4
    • Electrolyte panel
    Comments:
    Internal Use Only: {Notes for Internal Use}
    Internal Codes: Profile or single test
    Internal Laboratory: VP
    Internal Test Volume: N/A
    Cholesterol
    Suitable Sample:
    • PC
    • LH 
    • EDTA
    Unsuitable Sample:
    • CIT
    • FL
    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 4 hours

     

    Request/Test Included in:
    • Request test
    • CP2, 6
    • FP2, 5
    • AP2
    • Canine and feline liver monitoring panel
    Comments:
    Internal Use Only:
    • Do not perform on lipocleared samples.

     

    Internal Codes: Profile or single test
    Internal Laboratory: VP
    Internal Test Volume: 25 uL
    Cholinesterase
    Suitable Sample:
    • PC and EDTA
    Unsuitable Sample:
    • LH
    • CIT
    • FL
    Minimum Sample Size:
    • 1 mL whole blood (EDTA and serum) 
      Turnaround Time:
      • 5 working days

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Reduced nonspecific esterase activity in the blood is an indicator of exposure to organophosphates.

       

      Internal Use Only:
      • Send to QML
      • Run with a species normal control if possible 

       

      Internal Codes: X03
      Internal Laboratory: QML
      Internal Test Volume: N/A
      CK (Creatinine Kinase)
      Suitable Sample:
      • PC
      • LH
      Unsuitable Sample:
      • EDTA
      • FL
      Minimum Sample Size:
      • 2 mL whole blood

       

      Turnaround Time:
      • 4 hours

       

      Request/Test Included in:
      • Request test
      • CP2, 3, 6
      • FP 2, 5
      • EP 1, 2, 4, 6, 7
      • RP 1, 2
      • AP 2, 4
      Comments:
      • To assess muscle damage usually measure CK and AST.

       

      Internal Use Only: {Notes for Internal Use}
      Internal Codes: Profile, individual test
      Internal Laboratory: VP
      Internal Test Volume: 25 uL
      Clostridium botulinum toxin
      (Botulism (Clostridium botulinum))
      Suitable Sample:
      • Contact Vetpath

       

      Unsuitable Sample:
      Minimum Sample Size:
      Turnaround Time:
      • 5 working days

       

      Request/Test Included in:
      • Contact Vetpath for further information re sample requirements and availability.

       

      Comments:
      • The assay is a bioassay and its use has to be justifiable. It is not a sensitive test and is only suitable for species which may have large amounts of toxin. It is not suitable for species such as dogs if they are still alive as they will not have detectable amounts of toxin.

       

      Internal Use Only:
      Internal Codes:
      Internal Laboratory: Dep't of Agriculture.
      Internal Test Volume:
      Clotting Factors (non-Factor VIII)
      Suitable Sample:
      • CIT

       

      Unsuitable Sample:
      • EDTA
      • PC
      • LH

       

      Minimum Sample Size:
      • Contact Vetpath

       

      Turnaround Time:
      • Contact Vetpath re availablity (not run regularly) and transport requirements.

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Beware of insufficiently filled OR overfilled CIT tubes; it is important to fill exactly to the mark on the tubes.
      • Beware of expired tubes.
      • There is an information sheet available.
      Internal Use Only: {Notes for Internal Use}
      Internal Codes: N/A
      Internal Laboratory: University of Melbourne
      Internal Test Volume: N/A
      Coagulation Screen
      Suitable Sample:
      • CIT and EDTA

       

      Unsuitable Sample:
      • PC
      • LH
      Minimum Sample Size:
      • Filled CIT tube PLUS
      • 2 mL EDTA
      Turnaround Time:
      • 2 hours (less if urgent)

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Includes CBC, PT, PTT, fibrinogen, platelets and smear evaluation.
      • Beware of insufficiently filled OR overfilled CIT tubes; tubes must be filled to the line marked on the tube.
      • Beware of clots in the sample.
      Internal Use Only: {Notes for Internal Use}
      Internal Codes: H15
      Internal Laboratory: VP
      Internal Test Volume: N/A
      Coomb's Test (Canine)
      Suitable Sample:
      • EDTA
      • LH
      • CIT
      Unsuitable Sample:
      • PC
      • Samples from species other than dogs
      Minimum Sample Size:
      • 0.5 mL whole blood

       

      Turnaround Time:
      • Monday to Friday 3 hours, less if marked URGENT
      • Weekends and public holidays contact laboratory
      Request/Test Included in:
      • Request test

       

      Comments:
      • Only canine Coombs test is currently available

       

      Internal Use Only:
      • Switched to the DiaMed gel column system May 2007
      • Only canine tests available (so rarely do feline and equine that it is not worth keeping the reagents in stock)
      • Added the LH and CIT as suitable samples 28-4-09 as per kit insert (MMcC)
      Internal Codes: H09
      Internal Laboratory: VP
      Internal Test Volume: 400 uL of whole  EDTA-anticoagulated blood
      Copper (large animals)
      Suitable Sample:
      • LH
      • Tissue - liver if deficiency suspected
      • Tissue - liver or kidney if excess is suspected
      Unsuitable Sample:
      • PC
      • EDTA

       

      Minimum Sample Size:
      • 10 mL whole blood
      • 5 g fresh liver (can be frozen)
      Turnaround Time:
      • 2 weeks

       

      Request/Test Included in:
      • Request test

       

      Comments:
      Internal Use Only: {Notes for Internal Use}
      • Tissue goes to Ag dept
      • Requires 5g wet weight, could get away with 1g
      • cost to us $29.95, cost to our clients is $88 + GST
      Internal Codes: N/A
      Internal Laboratory: Dep't of Ag WA
      Internal Test Volume: 2 mL plasma
      Copper (small animals, birds)
      Suitable Sample:
      • LH
      • PC
      • Tissue (liver)
      Unsuitable Sample:
      • EDTA

       

      Minimum Sample Size:
      • 1 ml whole blood
      • 5g liver

       

      Turnaround Time:
      • Approximately 3 days

       

      Request/Test Included in:
      • Request test

       

      Comments:
      Internal Use Only:
      • Run 2 or 3 times a week (possibly Monday, but check)
      • tissue goes to Dept of Ag - require 5g liver, can get away with 1g.

      Cost to client is $155 + GST

      Internal Codes: N/A
      Internal Laboratory: WDP, Ag Dept
      Internal Test Volume: 250 uL serum or LH plasma
      Coronavirus - Direct IFA on Effusion Fluid for FIP
      Suitable Sample:
      • Abdominal or thoracic fluid, as fresh as possible.
      • EDTA (if not collected in EDTA, transfer to EDTA as soon as possible)
      • Immunohistochemistry can be done on formalin-fixed tissues
      • Keep sample chilled, not frozen
      Unsuitable Sample:
      • Serum or plasma

       

      Minimum Sample Size:
      • 1 mL fluid is the absolute minimum
      • 10 mL of fluid is preferable (extra fluid can be submitted in a plain tube without clot activator)
      Turnaround Time:
      • 5 working days (1-2 working days once received at the interstate referral laboratory)

       

      Request/Test Included in:
      • Request test (anti-FIP direct IFA)
      • May also be requested as an additional test to fluid cytology
      Comments: The anti-FCoV direct IFA test detects direct immunofluorescent staining of FIP-related antigens in macrophages in the effusion with anti-FCoV antibodies. This is a very specific diagnostic test as only FIP-infected cats will have virus-infected macrophages. This test has a specificity of 1.00 (100% positive predictive value) but a relatively low sensitivity of 0.75 (75%). No single test is able to confirm a diagnosis of FIP apart from histopathology or positive direct IFA test on effusion fluid. Clinical diagnosis of FIP should be based on a wide variety of tests including history, age, number of cats in household, persistent pyrexia and laboratory data including assessment of acute phase proteins, analysis of effusions, haematology and biochemical tests, as well as specific serological tests to list a few.
      Internal Use Only:
      Internal Codes: S29
      Internal Laboratory:
      • Uni of Sydney (Veterinary Pathology Diagnostic Services)
      • Ph 02 9351 7456
      • Fax 02 9351 7421
      Internal Test Volume:
      Coronavirus Antibody Titre by IFA (Feline)
      (Feline Coronavirus Antibody Titre by IFA)
      Suitable Sample:
      • PC (serum)
      • Feline only
      Unsuitable Sample:
      • EDTA
      • LH
      • Species other than cats
      Minimum Sample Size:
      • 1.0 mL whole blood

       

      Turnaround Time:
      • Run Tuesday and Friday
      • Cutoff time 7pm previous working week day 
      • Results available the same day
      Request/Test Included in:
      • This test is included in the FIP Screen
      • It may also be requested as an individual test (Coronavirus Indirect IFA)
      Comments: This is the gold-standard test for measurement of antibodies to feline coronavirus (FCoV).
      • Titres of 1:10 or less are considered seronegative
      • Titres 1:40 or greater considered seropositive
      • Titres <1:10 indicate a low likelihood of coronavirus shedding. If the cat is infected they are likely to stop shedding when isolated.
      • Cats with high titres (1:640 or higher) are almost always shedding virus but can stop shedding upon isolation. This can be demonstrated by a drop in their titre to low or undetectable levels.
      • Cats with clinical FIP usually have a high titre (1:640 or higher), although a few cases may show low titres in the terminal stages (particularly in the effusive forms).
      • The higher the titre the greater the specificity and sensitivity of this test for a diagnosis of FIP. The positive predictive index at 1:1600 is 0.94 and the negative predictive index 0.88 (J Vet Int Med 2003:17 781-790)
      • No single test is suitable to confirm a diagnosis of FIP apart from histopathology. Clinical diagnosis of FIP should be based on a wide variety of tests including history, age, number of cats in household, persistent pyrexia and laboratory data including assessment of acute phase proteins, analysis of effusions, haematology and biochemical tests as well as specific serological tests to list a few.
      Internal Use Only:
      • Serial dilutions are performed from 1:10 with 4-fold dilutions to 1:2560.
      • Although in theory this test can also be done on canine serum, this requires the use of specific canine reagent, plus there are no guidelines for interpretation of canine results, therefore TEST CANNOT BE DONE ON DOGS
      Internal Codes:
      • S23
      • S23L (for laboratories)
      • S23 (FIP Screen)
      • S23L (FIP Screen for laboratories) 
      • S16F (canine coronavirus Ab titre)
      Internal Laboratory: VP
      Internal Test Volume: 50 uL
      Cortisol (for ACTH stimulation test and LDDST)
      Suitable Sample:
      • PC

       

      Unsuitable Sample:
      • LH
      • EDTA
      • FL
      Minimum Sample Size:
      • 1.0 mL whole blood

       

      Turnaround Time:
      • Monday to Friday with a 2:00pm cutoff time
      • Saturdays with a 1:00pm cut off time
      • Results available same day (usually by 3:00pm)
      Request/Test Included in:
      • Cortisol
      • ACTH stimulation test (2 cortisol samples)
      • Low dose dexamethasone suppression test (3 cortisol samples)
      Comments:
      • A single cortisol is of minimal diagnostic value and dynamic tests of adrenal function are recommended for the diagnosis of both hypoadrenocorticism (Addison's disease) and hyperadrenocorticism (Cushing's disease).
      •  A client information sheet is available on these tests.
      Internal Use Only:
      • A client information sheet is available on these tests.
      • There is no specific information in the kit insert regarding samples other than serum to indicate that they are suitable or unsuitable.

       

      Internal Codes:
      • E01
      • E02
      • E03
      Internal Laboratory: VP
      Internal Test Volume: Minimum 110 uL
      CP1
      Suitable Sample:
      • EDTA
      • FL
      • PC
      Unsuitable Sample:
      Minimum Sample Size:
      • 1ml
      Turnaround Time:
      • 4 hours
      Request/Test Included in:
      • Request test
      Comments:
      • CP1 includes CBC,  ALT, ALP, GGT, bilirubin, urea, creatinine, amylase, glucose, total protein, albumin, globulins, and urine SG.
      Internal Use Only:
      Internal Codes:
      Internal Laboratory: VP
      Internal Test Volume:
      CP2
      Suitable Sample:
      • EDTA
      • FL
      • PC
      Unsuitable Sample:
      Minimum Sample Size:
      • 1ml in each tube
      Turnaround Time:
      • 4 hours
      Request/Test Included in:
      • Request test
      Comments:
      • CP2 includes CBC, CK, AST, ALT, ALP, GGT, bilirubin, urea, creatinine, lipase, amylase, glucose, cholesterol, Na, K, Cl, total protein, albumin, globulins, calcium, phosphorus, urine SG and ketones (if indicated).
      Internal Use Only:
      Internal Codes: CP2
      Internal Laboratory: VP
      Internal Test Volume:
      CP4 - Geriatric profile
      Suitable Sample:
      • EDTA
      • FL
      • PC
      Unsuitable Sample:
      Minimum Sample Size:
      • 1ml in each tube
      Turnaround Time:
      • 4 hours
      Request/Test Included in:
      • Request test
      Comments:
      • CP4 includes CBC, ALT, ALP, GGT, urea, creatinine, bilirubin, glucose, total protein, albumin, globulins, Na, K, Cl, urine SG. 
      Internal Use Only:
      Internal Codes: CP4
      Internal Laboratory: VP
      Internal Test Volume:
      CP6
      Suitable Sample:
      • EDTA
      • FL
      • PC
      Unsuitable Sample:
      Minimum Sample Size:
      • 1ml 
      Turnaround Time:
      • 4 hours
      Request/Test Included in:
      • Request test
      Comments:

      CP6 is the biochemistry panel of a CP2. This includes CK, AST, ALT, GGT. bilirubin, urea, creatinine, lipase, amylase, glucose, cholesterol, Na, K, Cl, total protein, albumin, globulin, calcium, phosphorus, urine SG and BOHB if indicated.

      Internal Use Only:
      Internal Codes: CP6
      Internal Laboratory: VP
      Internal Test Volume:
      C-Reactive Protein
      Suitable Sample:
      • PC
      Unsuitable Sample:
      • EDTA
      • LH
      Minimum Sample Size:
      • 2ml
      Turnaround Time:
      • 5 working days
      Request/Test Included in:
      • Request test
      Comments:

      This test is for dogs only.

      Internal Use Only:
      • Gribbles (ASAP currently unavailable)
      Internal Codes: B46RT
      Internal Laboratory: Gribbles
      Internal Test Volume:
      Creatinine
      Suitable Sample:
      • PC
      • LH
      • EDTA
      Unsuitable Sample:
      • FL

       

      Minimum Sample Size:
      • 2 mL whole blood

       

      Turnaround Time:
      • 4 hours

       

      Request/Test Included in:
      • Request test
      • CP1, 2, 3, 4, 5, 6
      • FP1, 2, 3, 4, 5
      • EP1, 2, 3, 6, 7
      • RP2, 4
      • Camelid profile
      • AP2, 4
      • Renal monitoring panel
      • Feline Geriatric panel
      Comments:
      Internal Use Only: FL samples have been used with this test with previous reagents, but there is no specific information provided with the Olympus reagents regarding the use of FL or CIT samples.
      Internal Codes: Profile or single test
      Internal Laboratory: VP
      Internal Test Volume: 50 uL
      Cross Match
      Suitable Sample:
      • EDTA & PC from both the donor and recipient

       

      Unsuitable Sample:
      • LH

       

      Minimum Sample Size:
      • 2 mL whole blood in PC tube from each of donor and recipient
        PLUS
      • 2 mL whole blood in an EDTA tube from each of donor and recipient
      Turnaround Time:
      • 2 hours (less if urgent)

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Avoid haemolysis as 2+ haemolysis gives invalid results.
      • Beware of agglutinated samples
      • Major cross match = donor RBCs + recipient serum
      • Minor cross match = recipient RBCs + donor serum
      Internal Use Only:
      • Plasma can be used instead of serum, but is undesirable in dogs as the presence of fibrinogen and other proteins increases rouleaux formation making interpretation more difficult.
      • In cats rouleaux formation is similar in both serum and plasma.
      Internal Codes:
      • H20
      • H21 (additional donors)
      Internal Laboratory: VP
      Internal Test Volume: N/A
      Cryptococcus Antigen Titre
      Suitable Sample:
      • PC
      • CSF
      Unsuitable Sample:
      • EDTA
      • LH
      Minimum Sample Size:
      • 2 mL whole blood
      • 100uL CSF

       

      Turnaround Time:
      • Run on Monday to Friday with a 3 pm cutoff for same day results.
      • Run on Saturday with a 1pm cut off for same day results.
      • Test takes approximately 3 hours to run.
      Request/Test Included in:
      • Request test

       

      Comments:
      • Test measures up to a titre of 1:65536.  If higher titrations are required, a second fee will be added to obtain the end point titre.
      • Positive predictive value in serum is >85%
      • Positive predictive value in CSF is >98%.
      • Samples MUST NOT be haemolysed for accurate results.  The laboratory will not be able to provide results for samples that are haemolysed.
      Internal Use Only:
      • S08latex - Cryptococcus latex agglutination

      • S08ep - for endpoint titration

      Internal Codes: S08 (see internal notes)
      Internal Laboratory: VP
      Internal Test Volume: N/A
      Cryptosporidium - RIM
      Suitable Sample:
      • Fresh faeces

       

      Unsuitable Sample:
      • Blood

       

      Minimum Sample Size:
      • Sufficient for smear

       

      Turnaround Time:
      • Same day

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • This test is designed to detect Cryptosporidium parvum in animal faeces
      • This test replaces the immunofluorescence (IFA) test
      • Reported sensitivity is 96.7%
      • Reported specificity is 99.9%
      Internal Use Only:
      • This test replaces the immunofluorescence (IFA) test
      • Test in use is the MegaCor FASTtest Cryptosporidium
      Internal Codes: P04 (cryptosporidium RIM)
      Internal Laboratory: VP
      Internal Test Volume: N/A
      Cryptosporidium - Smear Examination
      Suitable Sample:
      • Fresh faeces

       

      Unsuitable Sample:
      • Blood

       

      Minimum Sample Size:
      • Sufficient for smear

       

      Turnaround Time:
      • One working day (12 hours)

       

      Request/Test Included in:
      • Request test

       

      Comments:
      Internal Use Only: Is covered under staining in the staining manual.
      Internal Codes: P04
      Internal Laboratory: VP.
      Internal Test Volume: N/A
      CSF (Cerebrospinal Fluid) analysis and cytology
      Suitable Sample:
      • Sample in EDTA or special plain CSF tube (1-2 ml maximum needed from dogs & cats)
      • Contact the laboratory for special plain CSF tubes (without clot activator)
      • EDTA is suitable for cytology, but is not suitable for culture.
      • Do NOT use tubes with gel activator in them (usual red top plain clotted blood tubes).
      Unsuitable Sample:
      • LH

       

      Minimum Sample Size:
      • >100 uL
      • (preferably >0.5 mL)
      Turnaround Time:
      • < 3 hours (always treated as urgent)

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Should reach the laboratory within 2-4 hours, but if not possible keep cool in transit.
      • Place some CSF in an EDTA tube for better cell preservation.
      • Can add 10% autologous clear serum for longer transport but does not guarantee good cell preservation.
      Internal Use Only: {Notes for Internal Use}
      Internal Codes: C05
      Internal Laboratory: VP
      Internal Test Volume: Can do cytopsin only with eg 50 uL
      Culture & Sensitivity - Aerobic
      Suitable Sample:
      • Swab, fluid and/or fresh tissue
        PLUS
      • Air-dried smear for Gram stain
      Unsuitable Sample:
      • Dry swabs
      • Culture plates will not be accepted and will be disposed of if submitted.
      Minimum Sample Size:
      Turnaround Time:
      • Preliminary Gram stain: 4 hours (if needed).
      • Culture: 12-24 hours for most organisms (some take longer)
      • Sensitivity: 48 hours for most organisms (some take longer)
      Request/Test Included in:
      • Aerobic C&S
      • Premating swab
      • Mare swab
      Comments:
      • Samples should be stored in a cool, dry place; samples do not need to be refrigerated.
      • Samples stored at room temperature can be cultured for up to 3 days after collection.
      • Further sensitivities must be requested within 24 hours of the sensitivity report being sent out in order to obtain valid results.
      • Some in vitro resistance patterns can be predicted based on the organism cultured - this influences the drugs used in susceptibility testing and treatment.
      • Organisms that are intrinsically resistant to an antibiotic will be marked with an "X".
      • For information on intrinsic antibiotic resistance of specific bacateria, see the information sheet.
      Internal Use Only:
      • See comment added on "no plates to be submitted"
      • See comment added on "extra sensitivities within 24 hours"
      • Intrinsic Resistance link added 23-10-2008 (MMcC)
      Internal Codes:
      • M01
      • M01a (add-on to cytology)
      • M04
      Internal Laboratory: VP
      Internal Test Volume: N/A
      Culture & Sensitivity - Anaerobic & Aerobic
      Suitable Sample:
      • Swab, fluid and/or fresh tissue
        PLUS
      • Air-dried smear for Gram stain
        NOTE: Anaerobic culture is appropriate for aspirated fluid or tissues, faeces, surgically-collected samples.
      Unsuitable Sample:
      • Dry swabs
      • Swabs and surface samples are less suitable for anaerobic culture.
      • Culture plates will not be accepted and will be disposed of if submitted
      Minimum Sample Size:
      • 2 mL of aspirated fluid
      • At least 1 cm2 of tissue
      Turnaround Time:
      • Preliminary Gram stain: 4 hours (if needed)
      • Culture: 12-24 hours for most organisms (some take longer)
      • Sensitivity: 48 hours for most organisms (some take longer)
      Request/Test Included in:
      • Anaerobic C&S
      • Anaerobic culture is also included in non-healing wound culture
      Comments:
      • Samples should be stored in a cool, dry place.
      • Samples for anaerobic culture should not be refrigerated.
      • Samples stored at room temperature can be cultured for up to 3 days after collection.
      • Any extra sensitivies must be requested within 24 hours of the results being sent out in order to get meaningful results.
      • Some in vitro resistance patterns can be predicted based on the organism cultured - this influences the drugs used in susceptibility testing and treatment.
      • Organisms that are intrinsically resistant to an antibiotic will be marked with an "X".
      • For information on intrinsic antibiotic resistance of specific bacteria, see the information sheet.
      Internal Use Only:
      • See comment added on "no plates to be submitted"
      • See comment added on "extra sensitivities within 24 hours"
      • Link to sheet on intrinsic resistance of some bacteria added 23-10-2008
      Internal Codes:
      • M02
      • M02a (add-on to cytology)
      Internal Laboratory: VP
      Internal Test Volume: N/A
      Culture & sensitivity - Non-Healing Wound
      Suitable Sample:
      • Fluid and/or fresh tissue if preferred, but a swab can be submitted.
        PLUS
      • Air-dried smear for Gram stain
      Unsuitable Sample:
      • Dry swabs

       

      Minimum Sample Size:
      • For successful anaerobic culture, it is best to have 2 mL of aspirated fluid
        or
      • At least 1 cm2 of tissue.
      Turnaround Time:
      • Total turnaround is up to 4 weeks for acid-fast organisms.
      • Preliminary Gram stain: 4 hours (if needed).
      • Culture: 12-24 hours for most organisms (some take longer)
      • Sensitivity: 48 hours for most organisms (some take longer)
      Request/Test Included in:
      • Non-healing wound culture (NHW)

       

      Comments:
      • Includes aerobic and anaerobic culture, plus Z-N stain and reincubation for acid-fast organisms.
      • Samples should be stored in a cool, dry place and should not be refrigerated.
      • Do not freeze tissue for culture.
      • Samples stored at room temperature can be cultured for up to 3 days after collection.
      • Some in vitro resistance patterns can be predicted based on the organism cultured - this influences the drugs used in susceptibility testing and treatment.
      • Organisms that are intrinsically resistant to an antibiotic will be marked with an "X".
      • For information on intrinsic antibiotic resistance of specific bacteria, see the information sheet
      Internal Use Only:
      Internal Codes:
      • M03
      • C25 (add-on to cytology)
      Internal Laboratory: VP
      Internal Test Volume:
      Cyclosporin
      Suitable Sample:
      • EDTA, needs to be fresh as possible

       

      Unsuitable Sample:
      • PC
      • LH
      Minimum Sample Size:
      • 0.5ml whole EDTA

       

      Turnaround Time:
      • 2 working days

       

      Request/Test Included in:
      • Request test 
      Comments:
      • Blood cyclosporine levels are usually checked a day or two into therapy and then every two to four weeks.
      Internal Use Only:
      • DO NOT SEPARATE
      • Run goes on at 11am and 3pm Monday to Friday, 11am only Saturday and Sunday
      • Mark sample urgent.  Must go to PathWest same day as arrives.
      Internal Codes: X18
      Internal Laboratory: PathWest Biochemistry, ph 9346 2093
      Internal Test Volume: 100 uL (prefer 0.5ml) whole EDTA
      Cytology - ear
      (Ear Cytology)
      Suitable Sample:
      • Air dried smears for cytology
      • Swab for culture if required
      Unsuitable Sample:
      Minimum Sample Size:
      • Single smear

       

      Turnaround Time:
      • 24 hours (Mon-Sat)

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Cellular preservation for cytology is superior on an air dried smear.

       

      Internal Use Only:
      Internal Codes:
      • C15
      • C16
      Internal Laboratory: VP
      Internal Test Volume:
      Cytology - eye
      (Eye Cytology)
      Suitable Sample:
      • Air dried smears for cytology
      • Swab (for culture; less suitable for cytology)
      Unsuitable Sample:
      Minimum Sample Size:
      • Single smear

       

      Turnaround Time:
      • 24 hours (Mon-Sat)

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • Cellular preservation is better on an air dried smear.

       

      Internal Use Only:
      Internal Codes:
      • C15
      • C16
      Internal Laboratory: VP
      Internal Test Volume:
      Cytology - Fine needle aspirate (FNA)
      (Fine Needle Aspirate Cytology)
      Suitable Sample:
      • Air-dried smears from fine needle aspirate (FNA)
      • Impression smears from tissue biopsy
      Unsuitable Sample:
      Minimum Sample Size:
      • Single smear

       

      Turnaround Time:
      • 24 hours Monday-Saturday
      • Most results are available later the same day
      • Samples submitted late in the day may be read the next morning (results available by 1 pm)
      • If a sample is urgent, results can be available within 4 hours 
      Request/Test Included in:
      • Request test

       

      Comments:
      • Submission of at least two smears or smears from more than one FNA of a lesion increases the chances of a diagnostic sample without increasing the cost
      • Multiple aspirates from multiple lymph nodes are charged as a single sample
      • Extra aspirates from separate lesions in the same animal are charged at a reduced rate
      • Repeat aspirates from a lesion submitted within 1 week of the original submission are charged at a reduced rate
      Internal Use Only:
      Internal Codes:
      • C01
      • C01h
      • C02
      Internal Laboratory: VP
      Internal Test Volume:
      Cytology - fluid/body cavity effusion
      (Fluid Analysis & Cytology)
      Suitable Sample:
      • Fresh smears made at the time of collection are useful.
      • EDTA is optimal for cytology.
      • Collect non-anticoagulated if sample is to be cultured; samples in EDTA are not suitable for culture.
      Unsuitable Sample:
      • LH

       

      Minimum Sample Size:
      • 0.5 mL if cell count is to be done
      • Smears for cytology only
      Turnaround Time:
      • 24 hours Monday-Saturday
      • Most results are available later the same day
      • Samples submitted late in the day may be read the next morning (results available by 1 pm)
      Request/Test Included in:
      • Request test

       

      Comments:
      • If sufficient fluid, place some in an EDTA tube and the rest in either a PC tube (without activator) or a sterile urine container.

       

      Internal Use Only:
      Internal Codes:
      • C03
      • C03a (second site, same animal)
      Internal Laboratory: VP
      Internal Test Volume: 0.5 mL