Test Information

To locate information on a particular test, click on the relevant letter and follow the trail. eg To find information on sample requirements, test availability and turnaround time for FeLV, click on "F" and then click on the relevant test (FeLV soluble Ag or FeLV IFA).

To download a copy of our lab request form please click link below:

LAB REQUEST FORM


Ear Cytology
(Cytology - ear)
Suitable Sample:
  • Air dried smears for cytology
  • Swab (if culture requested; less suitable for cytology)
Unsuitable Sample:
Minimum Sample Size:
  • Single smear

 

Turnaround Time:
  •  24 hours (Mon-Sat)

 

Request/Test Included in:
  • Request test

 

Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes:
  • C15
  • C16
Internal Laboratory: VP
Internal Test Volume:
eCG (Equine Chorionic Gonadotrophin, PMSG) ELISA
(PMSG (Pregnant Mare Serum Gonadotrophin, equine chorionic gonadotrophin) - ELISA)
Suitable Sample:
  • PC preferred
  • LH plasma can be used
Unsuitable Sample:
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run once a week (Wednesday)
  • Results available by Thursday am
Request/Test Included in:
  • Request test
  • All PMSGs are the ELISA test
  • The rapid RIM test is no longer available.
Comments:
  • Mare pregnancy 45 - 120 days
  • Optimum 70 days
Internal Use Only:
  • Use of the rapid RIM test was stopped 28-6-07 due to poor sensitivity
  • This has been replaced by the ELISA which is run once a week when there are samples to be done.
Internal Codes: E12
Internal Laboratory: VP
Internal Test Volume: 250 uL serum (two duplicates of 100 uL each)
Egg Count - Faecal (single & pooled)
(Faecal Egg Count)
Suitable Sample:
  • Fresh faeces

 

Unsuitable Sample:
  • Faecal swab

 

Minimum Sample Size:
  • 2-3 gm (a larger volume such as 10 gm is better)

 

Turnaround Time:
  • 4 hours, Monday to Saturday

 

Request/Test Included in:
  • Request test

 

Comments:
  • May be done on either individual animals or a pooled samples.
  • A pooled sample may contain faeces from a maximum of 10 animals.
  • The saturated salt solution is efficient at floating common parasite eggs, but does not float trematode eggs and some tapeworm eggs. Specific procedures are required for these parasites.
  • Faeces are stable for 3 days when refrigerated.
Internal Use Only:
Internal Codes:
  • P05
  • P06
  • P07
Internal Laboratory: VP
Internal Test Volume:
Ehrlichia canis (AAHL)
Suitable Sample:
  • PC
Unsuitable Sample:
  • EDTA, LH

 

Minimum Sample Size:
  • 2 - 3 mL whole blood

 

Turnaround Time:
  • 10-14 days (contact laboratory)

 

Request/Test Included in:
  • Request test

 

Comments:
  • Test is run weekly and takes a week to complete.
Internal Use Only:
  • Run on Wednesday, results are due to the following Wednesday.
  • Samples must reach Vetpath the Monday before the test is run.
Internal Codes:
Internal Laboratory: AAHL Geelong
Internal Test Volume: 1 mL clear serum
Ehrlichia canis (Vetpath)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run Monday & Thursday
  • Cutoff time 7pm previous working weekday
  • Results late that day
Request/Test Included in:
  • Request test

 

Comments:
  • Test is run weekly
  • Store sample at 2-8oC
  • IFAT
Internal Use Only:
  • Samples must reach Vetpath the day before the test is run.
  • Test is also available via AAHL (file still exists, but is not linked)
Internal Codes:
  • Q13
  • Q13i
  • Q13L
Internal Laboratory: Vetpath
Internal Test Volume:
EIA (Equine Infectious Anaemia; Coggins test)
(Equine Infectious Anaemia (EIA) - Antibody Titre)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • LH
  • EDTA
Minimum Sample Size:
  • 4 mL whole blood

 

Turnaround Time:
  • 3 working days

 

Request/Test Included in:
  • Request test
  • Can also be done with EVA (equine viral arteritis)
Comments:
  • Agar gel immunodiffusion test

 

Internal Use Only: Need to confirm price with Dep't of Ag if not in list
Internal Codes:
  • SA1
  • SA31 (combined EIA and EVA)
Internal Laboratory: Dept of Ag, WA
Internal Test Volume:
Electrolyte Panel
(Monitoring panel - electrolytes)
Suitable Sample:
  • PC
  • LH (more accurate for electrolytes, but may not suitable if wish to add other tests, especially serology tests)
Unsuitable Sample:
  • EDTA
  • SC
  • FL
Minimum Sample Size:
  • 2 mL whole blood
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
Comments:
  • Includes Na, K, Cl and Na:K ratio
Internal Use Only:
Internal Codes: B44
Internal Laboratory: VP
Internal Test Volume:
Electronmicroscopy (EM) of Faeces
Suitable Sample:
  • Faeces

 

Unsuitable Sample:
  • Blood

 

Minimum Sample Size:
Turnaround Time:
  • A few weeks

 

Request/Test Included in:
  • Request test

 

Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes: F10
Internal Laboratory: Dept of Ag, WA
Internal Test Volume:
Electrophoresis - Serum protein
(Serum protein electrophoresis)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • LH
  • EDTA
  • Haemolysed samples
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 5 working days

 

Request/Test Included in:
  • Request test

 

Comments:
  • Used primarily to differentiate polyclonal from monoclonal gammopathies.
  • Samples must be non-haemolysed.

 

Internal Use Only:
  • Now done at WDP
  • Need to get them to sent the actual tracing
  • If need to make changes to the band marking, just mark it and send it back and they will reintegrate the numbers
  • Need to verify cost et
Samples that are haemolysed must not be sent.

If the sample is haemolysed then a new sample must be requested.
Internal Codes: B19
Internal Laboratory: WDP .... need to check on any specific requirements
Internal Test Volume: 200uL of serum is adequate, but preferably more.
Electrophoresis - Urine
(Urine electrophoresis)
Suitable Sample:
  • Urine
Unsuitable Sample:
  • Blood
Minimum Sample Size:
  • 5ml urine
Turnaround Time:
  • 5 working days
Request/Test Included in:
  • Request test
Comments:
  • Urine electrophoresis is a screening test to detect monoclonal protein in the urine.
  • A narrow based peak in the urine electrophoresis suggests the presence of Bence Jones proteinuria due to multiple myeloma.
Internal Use Only:
Internal Codes: B19
Internal Laboratory: WDP
Internal Test Volume:
Encephalitozoon Cuniculi (IFA)
Suitable Sample:
  • Plain clotted
Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 1ml whole blood
Turnaround Time:
  • Run Mondays and Thursdays
  • Cut-off time is 7pm the previous working weekday
Request/Test Included in:
  • Request test
Comments:
Internal Use Only:
Internal Codes: S34
Internal Laboratory: VP
Internal Test Volume:
Encephalitozoon Cuniculi (PCR)
(no longer available)
Suitable Sample:
  • Serum
Unsuitable Sample:
  • EDTA
  • LH
  • Urine
Minimum Sample Size:
  • 1ml plain clotted sample

 

Turnaround Time:
  • Up to 10 days
Request/Test Included in:
  • Request test
Comments:
  • PCR
Internal Use Only:
Internal Codes: G20
Internal Laboratory:
Internal Test Volume:
Endocrinology panel - Equine
(Equine endocrinology panel)
Suitable Sample:
  • EDTA plus PC plus FL
Unsuitable Sample:
  • LH
  • Urine
Minimum Sample Size:
  • 2ml EDTA
  • 2ml PC
  • 2ml FL
Turnaround Time:
  • 24 hours for endogenous ACTH and glucose
  • 2 days for insulin
Request/Test Included in:
  • Request test
Comments:
  • Panel includes endogenous ACTH, insulin and glucose concentrations.
Internal Use Only:
Internal Codes: EECUSH
Internal Laboratory: VP
Internal Test Volume:
EP2
Suitable Sample:
  • EDTA
  • PC
  • FL
Unsuitable Sample:
Minimum Sample Size:
  • 1ml in each tube
Turnaround Time:
  • 4 hours
Request/Test Included in:

Request test

Comments:

EP2 includes a CBC, CK, AST, ALP, GGT, bilirubin, urea, creatinine, total protein, albumin, globulin, Na, K, Cl.

Internal Use Only:
Internal Codes: EP2
Internal Laboratory: VP
Internal Test Volume:
EP3
Suitable Sample:
  • EDTA
  • PC
  • FL
Unsuitable Sample:
Minimum Sample Size:
  • 1ml in each tube
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
Comments:
  • EP3 includes CBC, CK, AST, ALP, GGT, GLDH, bilirubin, triglycerides, glucose, urea, creatinine, total protain, albumin, globulins, Na, K, Cl, calcium and phosphorus.
Internal Use Only:
Internal Codes: EP3
Internal Laboratory: VP
Internal Test Volume:
Equine Chorionic Gonadotrophin (eCG, PMSG) ELISA
(PMSG (Pregnant Mare Serum Gonadotrophin, equine chorionic gonadotrophin)
Suitable Sample:
  • PC preferred
  • LH plasma can be used
Unsuitable Sample:
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run once a week (Wednesday)
  • Results available by Thursday am
Request/Test Included in:
  • Request test
  • All PMSGs are the ELISA test
  • The rapid RIM test is no longer available.
Comments:
  • Mare pregnancy 45 - 120 days
  • Optimum 70 days
Internal Use Only:
  • Use of the rapid RIM test was stopped 28-6-07 due to poor sensitivity
  • This has been replaced by the ELISA which is run once a week when there are samples to be done.
Internal Codes: E12
Internal Laboratory: VP
Internal Test Volume: 250 uL serum (two duplicates of 100 uL each)
Equine Cushing's/Metabolic Syndrome Screen (Endocrinology panel)
Suitable Sample:
  • EDTA
  • PC
  • FL
Unsuitable Sample:
  • LH
Minimum Sample Size:
  • 2ml in each tube
Turnaround Time:
  • 8 hours
Request/Test Included in:
  • Request test
Comments:
  • Includes glucose, insulin and endogenous ACTH
Internal Use Only:
Internal Codes: Eecush
Internal Laboratory: VP
Internal Test Volume:
Equine Glucose Tolerance Panel
Suitable Sample:
  • EDTA and PC (x2)
Unsuitable Sample:
  • Urine
Minimum Sample Size:
  • 2ml whole blood 
Turnaround Time:
  • 8 hours
Request/Test Included in:
  • Request test
Comments: The Equine Glucose Tolerance Panel includes:
  • Endogenous ACTH
  • 2 x insulin and 2 x glucose (pre- and post-glucose administration)
Internal Use Only:
Internal Codes: EGTP
Internal Laboratory: VP
Internal Test Volume:
Equine health screen
Suitable Sample:
  • EDTA and PC and FL blood tubes
Unsuitable Sample:
  • LH can be used for some biochemical tests
Minimum Sample Size:
  • 2ml whole blood
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
Comments:
  • Includes CBC, ALP, GGT, GLDH, albumin, triglycerides, glucose, bilirubin, serum bile acids, endogenous ACTH and insulin.
Internal Use Only:
Internal Codes: EQHS
Internal Laboratory: VP
Internal Test Volume:
Equine Herpesvirus (EHV-1 and 4) detection by PCR
(Herpesvirus - Equine (EHV-1 and 4) detection by PCR)
Suitable Sample:
  • Nasal swab not in culture media
Unsuitable Sample:
  • Fixed tissue
  • Frozen tissue
  • Blood
Minimum Sample Size:
  • Nasal swab not in culture media
Turnaround Time:
  • Allow at least 10 working days
    Request/Test Included in:
    • Detection of EHV-1 and 4 by PCR
      Comments:
      Internal Use Only:
      Internal Codes: SV4
      Internal Laboratory: Ag Dept
      Internal Test Volume:
      Equine Herpesvirus (EHV1) Antibody Titre
      (Herpesvirus - Equine (EHV1) Antibody Titres)
      Suitable Sample:
      • PC
      • If the sample is the first of a pair, please indicate this on the submission form so that the sample can be held and the pair processed together.
      Unsuitable Sample:
      •  LH
      • EDTA
      Minimum Sample Size:
      • Minimum 4 mL whole blood

       

      Turnaround Time:
      • Up to 3 weeks
      Request/Test Included in:
      • Request test
      • It is essential to indicate if this is a single sample, or the first/second of a pair.  All paired samples must be processed at the same time, even if the first of the pair has already been processed.
      Comments:
      • For detection of of a rise in Ab (indicative of current infection) paired sera (acute and convalescent) are required 14 days apart.
      • Paired samples must be processed at the same time; the first of the pair needs to be separated and frozen until the second sample arrives.  If the first of the pair has already been processed, it will need to re-tested with the second sample (ie it would need to be run, and charged for, twice).
      • Store whole blood at 4oC, but if transport is delayed it is necessary to separate the serum from the cells.
      Internal Use Only:
      • Now goes to the Ag Dept NOT Uni of Melbourne
      • Send separated if the sample will not be sent within 36 hours.
      • Transport cool (on ice).
      • Can be frozen.
      Internal Codes: SV2 (single) SV3 (paired)
      Internal Laboratory: AgWA
      Internal Test Volume: 4ml whole blood
      Equine Infectious Anaemia (EIA) - Antibody Titre (Coggins Test)
      (EIA - Antibody Titre; Coggins Test)
      Suitable Sample:
      • PC

       

      Unsuitable Sample:
      • LH
      • EDTA
      Minimum Sample Size:
      • 4 mL whole blood

       

      Turnaround Time:
      • 3 working days

       

      Request/Test Included in:
      • Request test
      • Can also be done with EVA (equine viral arteritis)
      Comments:
      • Agar gel immunodiffusion test

       

      Internal Use Only: Need to confirm price with Dep't of Ag if not in list
      Internal Codes:
      • SA1
      • SA31 (combined EIA and EVA)
      Internal Laboratory: Dept of Ag, WA
      Internal Test Volume:
      Equine respiratory PCR panel
      Respiratory PCR panel - Equine
      Suitable Sample:
      • BAL/TW in EDTA
      • Dry swab
      Unsuitable Sample:
      • Blood
      Minimum Sample Size:
      • 1ml fluid
      Turnaround Time:
      • 3 working days
      Request/Test Included in:
      • Request test
      Comments:

      Panel includes:

      • Equine Herpesvirus 1
      • Equine Herpesvirus 4
      • Rhodococcus equi
      • Streptococcus equi subsp. equi
      • Chlamydia psittaci
      Internal Use Only:
      Internal Codes: G32
      Internal Laboratory: Vetnostics
      Internal Test Volume:
      Equine Viral Arteritis (EVA) Antibody Titre
      (EVA antibody titre)
      Suitable Sample:
      • PC

       

      Unsuitable Sample:
      • LH
      • EDTA
      Minimum Sample Size:
      • 4 mL whole blood

       

      Turnaround Time:
      • Must be at Vetpath before Wednesday 11am.
      • Results for samples received by this time should be available Wednesday of the following week.
      • If there are problems with the test it may need to be repeated.
      Request/Test Included in:
      • Request test
      • May be done in conjunction with EIA (equine infectious anaemia)
      Comments:
      • Serum virus neutralisation test

       

      Internal Use Only:
      • Samples must be at the Dep't of Ag by Wednesday afternoon for Thursday setup.
      • The test is only set up on Thursdays and is read the following Tuesday if all goes well (they check the cell line on Friday).
      Internal Codes:
      • SA3
      • SA31 (combined EVA and EIA)
      Internal Laboratory: Dept of Ag, WA (current information provided by Parkinson)
      Internal Test Volume:
      Erythropoietin
      Suitable Sample:
      • PC
      Unsuitable Sample:
      • EDTA
      • LH
      • FL
      • CIT
      Minimum Sample Size:
      • 2ml whole blood
      Turnaround Time:
      • 3 days
      Request/Test Included in:
      • Request test (see comments)
      Comments:
      • Must submit a patient and control sample
      • Take the samples between 7:30am and 12:00pm and let clot at room temperature
      • Must be separated within 60 minutes
      • Serum is stable at 2 - 8oC for 7 days
      Internal Use Only:
      • Submit to RPH
      • Cost to client is $112.50 + GST

       

      Internal Codes:
      Internal Laboratory: RPH
      Internal Test Volume: 1ml serum
      ESR (Erythocyte Sedimentation Rate)
      (no longer available)
      Suitable Sample:
      • EDTA
      • LH (but not optimal)
      Unsuitable Sample:
      • PC
      Minimum Sample Size:
      • 2 mL whole blood
      Turnaround Time: 2 hours
      Request/Test Included in:
      • Request test
      • These are not done as part of routine CBC or equine profiles, but can be added at no extra cost at the client's request.
      Comments:
      • LH may be inaccurate
      • This test in no longer available
      Internal Use Only:
      • Only done on equine samples
      • From 01-05-2007 are only done upon request
      • No longer available as of 20/10/08 as per DB
      Internal Codes:
      Internal Laboratory: VP
      Internal Test Volume:
      Estrone sulphate ELISA (quantitative)
      (Oestrone Sulphate)
      Suitable Sample:
      • PC
      • EDTA
      Unsuitable Sample:
      • Haemolysed samples
      • Lipaemic samples
      Minimum Sample Size:
      • 2 mL whole blood

       

      Turnaround Time:
      • Test is done on Wednesdays (cutoff time 11:00 am)
      • Results are available by noon on Thursday at the latest
      Request/Test Included in:
      • Request test

       

      Comments:
      1. Samples should be separated and the serum/plasma stored in the freezer once it reaches Vetpath.
      2. Oestrone (estrone) sulphate is a steroid hormone detectable in the circulation in many species. It can be produced in the gonads, the adrenals and is produced by the foetal-placental unit during pregnancy.
      3. Pregnancy >80 days: Oestrone sulphate levels rise during pregnancy and are a good indicator of a viable pregnancy. In mares oestrone sulphate is detectable in the maternal circulation from early as 60 days post-mating until foaling. In the event of foetal death oestrone sulphate disappears rapidly from circulation.
      4. Cryptorchidism in horses 3 yo or older. See information sheet.
      5. Goat pregnancy >50 days post-service
      Internal Use Only: Use the ELISA for all stallion/cryptorchid and non-equine samples

      All pregnancy samples are to be done by ELISA on the following Wednesday unless:
      1. The sample is stamped "urgent".
      2. The pathologist requests the rapid test is to be used.
      3. If there is only one sample for the week and it is a pregnancy test, the scientist should ask Sue/Mary/John and it may be decided to use the rapid test.
      Internal Codes:
      • E11E
      • E11EL (for laboratories)
      Internal Laboratory: VP
      Internal Test Volume:
      Estrus cytology
      (Oestrus Cytology/Vaginal cytology)
      Suitable Sample:
      • Air-dried smear(s)
        OR
      • Swab (not ideal for cellular preservation)
      Unsuitable Sample:
      Minimum Sample Size:
      • Single smear

       

      Turnaround Time:
      • <24 hours Mon-Sat

       

      Request/Test Included in:
      • Request test

       

      Comments:
      • May be used in conjunction with serum progesterone

       

      Internal Use Only:
      Internal Codes:
      • C16
      • C17
      Internal Laboratory: VP
      Internal Test Volume:
      EVA
      (Equine Viral Arteritis Antibody Titre)
      Suitable Sample:
      • PC

       

      Unsuitable Sample:
      • LH
      • EDTA
      Minimum Sample Size:
      • 4 mL whole blood

       

      Turnaround Time:
      • Must be at Vetpath before Wednesday 11am.
      • Results for samples received by this time should be available Wednesday of the following week.
      • If there are problems with the test it may need to be repeated.
      Request/Test Included in:
      • Request test
      • May be done in conjunction with EIA (equine infectious anaemia)
      Comments:
      • Serum virus neutralisation test

       

      Internal Use Only:
      • Samples must be at the Dep't of Ag by Wednesday afternoon for Thursday setup.
      • The test is only set up on Thursdays and is read the following Tuesday if all goes well (they check the cell line on Friday).
      Internal Codes:
      • SA3
      • SA31 (combined EVA and EIA)
      Internal Laboratory: Dept of Ag, WA (current information provided by Parkinson)
      Internal Test Volume:
      Eye Cytology
      (Cytology - eye)
      Suitable Sample:
      • Air dried smears
      • Swab
      Unsuitable Sample:
      Minimum Sample Size:
      • Single smear

       

      Turnaround Time:
      •  24 hours (Mon-Sat)

       

      Request/Test Included in:
      • Request test

       

      Comments:
      Internal Use Only: {Notes for Internal Use}
      Internal Codes:
      • C15
      • C16
      Internal Laboratory: VP
      Internal Test Volume: