Test Information

To locate information on a particular test, click on the relevant letter and follow the trail. eg To find information on sample requirements, test availability and turnaround time for FeLV, click on "F" and then click on the relevant test (FeLV soluble Ag or FeLV IFA).

To download a copy of our lab request form please click link below:

Lab Request Form


Pancreas specific lipase SNAP (Canine)
(Canine Pancreas specific Lipase SNAP)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
  • FL
Minimum Sample Size:
  • 1 mL whole blood if submitted as a stand-alone test

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Request test
  • Make sure that cPL is requested, not just lipase
Comments:
  • The DGGR lipase activity (in the biochemistry panel) has been found to have excellent agreement with the Spec cPL. 
    The test is canine-specific and cannot be used for cats.
  • This is a qualitative test (SNAP test) that gives a "positive" or "negative" result.
  • This test is reported to have 94-97.4% agreement with the Spec cPL test which is the quantative test.
Internal Use Only: This is a test for in-house use, it is different to the quantitative Spec cPL test that is done at the Idexx laboratory (not available to VETPATH)
Internal Codes:
  • B26 as stand-alone test
  • B26a as an add-on test
Internal Laboratory: VP
Internal Test Volume: Approximately 50 uL serum (3 drops)
Pancreas specific lipase SNAP (Feline)
(Feline pancreas specific lipase SNAP)
Suitable Sample:
  • PC
Unsuitable Sample:
  • EDTA
  • LH
  • FL
Minimum Sample Size:
  • 2ml whole blood
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request SNAP fPL
Comments:
  • The DGGR lipase activity (now in the biochemistry panel) has been found to have excellent agreement with the Spec fPL. 

  • SNAP test has 82 - 96% agreement with the quantitative spec fPL test.

  • The sensitivity of the Spec fPL test is 79% for diagnosing pancreatitis in cats and a specificity is 80% for ruling out pancreatitis. 
Internal Use Only:
Internal Codes: B27FI
Internal Laboratory: VP
Internal Test Volume:
Pancreas specific lipase Spec - Canine
(Lipase - canine pancreatic spec lipase)
Suitable Sample:
  • PC
Unsuitable Sample:
  • EDTA
  • LH
  • FL
Minimum Sample Size:
  • 2ml whole blood
Turnaround Time:
  • 5 working days
Request/Test Included in:
  • Request test (must specify Spec canine pancreatic lipase)
Comments:
Internal Use Only:
Internal Codes: B27C
Internal Laboratory: Idexx
Internal Test Volume:
Pancreas specific lipase Spec (feline)
(Feline pancreatic specific lipase Spec)
Suitable Sample:
  • PC
Unsuitable Sample:
  • EDTA
  • LH
  • FL
Minimum Sample Size:
  • 2ml whole blood
Turnaround Time:
  • 3 working days after arriving at referral laboratory
  • Allow 5 working days from submission to Vetpath
Request/Test Included in:
  • Request test (must specify feline pancreas specific lipase)
Comments:
  • Pancreatitis is reported to be a common finding at post-mortem in cats
    (67%), but can be very difficult to diagnose clinically.
  • Clinical pancreatitis in cats occurs with a wide range of conditions including diabetes mellitus, inflammatory bowel disease, cholangiohepatitis and hepatic lipidosis.
  • Serum amylase and total lipase are insensitive and have not been found useful in the diagnosis of pancreatitis in cats.
  • Specific feline pancreatic lipase is reported to have a sensitivity of 100% in moderate to severe cases of feline pancreatitis and a sensitivity of 45% in mild cases.
  • Specific feline pancreatic lipase is reported to have a specificity of 100% in healthy cats and a specificity of 67% in symptomatic cats with histologically normal pancreata.
Internal Use Only:
Internal Codes: B27F
Internal Laboratory:
Internal Test Volume:
Panleukopaenia (feline) Antibody Titre
(Feline Panleukopaenia Virus Antibody Titre by HI)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
  • CIT
  • FL
Minimum Sample Size:
  • 3 ml whole blood

 

Turnaround Time:
  • Approximately 2 weeks

 

Request/Test Included in:
  • Request test (must specifically request test by haemagglutination inhibition)
  • Contact the laboratory if doing multiple tests for a client in the same submission
Comments:
  • HI = HAI = Haemagglutination inhibition test
  • If not specifically requested, the panleukopenia antibody titre will be done at VP by IFA
  • Panleukopenia is also called feline infectious enteritis
Internal Use Only: Samples are sent to Scotland on Mondays and results are returned within two weeks.
Internal Codes:
  • S25B
  • S25B+ (additional tests for same client in same batch)
Internal Laboratory: Biobest, Scotland
Internal Test Volume: 1 ml clear serum
Paracetamol
Suitable Sample:
  • LH
  • PC
Unsuitable Sample:
  • EDTA

 

Minimum Sample Size:
  • 0.5 - 1 mL PC whole blood
  • 0.5 mL LH whole blood
Turnaround Time:
  • Done daily Mon-Fri

 

Request/Test Included in:
Comments:
  • Results available same day Mon-Fri if received early in the day, otherwise may be delayed until the next day
  • Check for weekend availability
Internal Use Only: {Notes for Internal Use}
Internal Codes: X02
Internal Laboratory: WDP Joondalup
Internal Test Volume: 100 uL serum OR LH plasma
Parasite Identification
Suitable Sample:
  • Parasite
  • Faeces
Unsuitable Sample:
  • Blood

 

Minimum Sample Size:
  • Single parasite

 

Turnaround Time:
  • 1 week

 

Request/Test Included in:
  • Request test

 

Comments:
  • Not available Christmas to New Year

 

Internal Use Only: {Notes for Internal Use}
Internal Codes: P25
Internal Laboratory: Murdoch University Parasitology
Internal Test Volume:
Parasites - blood smear evaluation
(Blood Parasites)
Suitable Sample:
  • Air-dried blood smear
  • EDTA blood
Unsuitable Sample:
  • PC

 

Minimum Sample Size:
  • 0.5 mL if submitting blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Request test

 

Comments:
  • If looking for Mycoplasma haemofelis (Haemobartonella felis) then capillary blood (no anticoagulant) is most likely to yield positive results in infected cats.

 

Internal Use Only:
Internal Codes: P01
Internal Laboratory: VP
Internal Test Volume: Blood smear
Parathyroid hormone (PTH)
(PTH)
Suitable Sample:
PC
Unsuitable Sample:
  • EDTA
  • LH
  • Citrate
Minimum Sample Size:
  • At least 2.0 mL whole blood
  • Submit an extra serum sample for measurement of calcium concentration

 

Turnaround Time:
  • At least 10 - 14 working weekdays

 

Request/Test Included in:
  • Request Test, contact laboratory in advance.

 

Comments:
  • Sample must be centrifuged within 1 - 2 hours of collection.
  • A second sample must be submitted if ionised calcium is required.
    • Contact the laboratory to discuss with a pathologist before submission of the sample.
Internal Use Only:

Send to Michigan state university

Internal Codes: E36R
Internal Laboratory: MSU
Internal Test Volume: 1 ml serum
Parathyroid-related protein (PTHrp)
Parathyroid-related protein (PTHrp)
Suitable Sample:
  • EDTA
Unsuitable Sample:
  • PC
Minimum Sample Size:
  • 3ml whole blood
Turnaround Time:
  • 10 - 14 working days
Request/Test Included in:
  • Request test, contact lab to get a current price.
Comments:
    • Contact the laboratory to discuss with a pathologist before submission of the sample.
  • Samples must be centrifuged within the first hour.
  • Sample should not be lipaemic (fasted).
  • Sample must be sent frozen.
Internal Use Only:

Send plasma frozen

Internal Codes: E36aR
Internal Laboratory: MSU
Internal Test Volume:
Parentage Testing
Suitable Sample:
  • See comments

 

Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
Request/Test Included in:
Comments:
Internal Use Only: They will not deal via diagnostic laboratories.
Internal Codes:
Internal Laboratory:
Internal Test Volume:
PARR - Lymphoma
Suitable Sample:
  • Blood
  • Cytology smears
  • Fluid
Unsuitable Sample:
Minimum Sample Size:
  • Must have 50 000 cells
Turnaround Time:
  • 2 - 3 weeks
Request/Test Included in:
  • Request test
Comments:
Internal Use Only:
Internal Codes: T30
Internal Laboratory: Colorado State University
Internal Test Volume:
Partial Thromboplastin Time
(PTT, aPTT)
Suitable Sample:
  • CIT

 

Unsuitable Sample:
  • PC
  • LH
  • EDTA
Minimum Sample Size:
  • Filled CIT tube for PT only
    PLUS
  • 2 mL EDTA if doing coagulation profile
Turnaround Time:
  • 2 hours (less if urgent)

 

Request/Test Included in:
  • Request PT
  • Request PT/PTT
  • Coagulation screen

Comments:
  • Beware of insufficiently filled OR overfilled CIT tubes; tubes must be filled to just the line marked on the tube.
  • Beware of clots in the sample.
Internal Use Only: {Notes for Internal Use}
Internal Codes:
  • H11
  • H12
  • H15
Internal Laboratory: VP
Internal Test Volume: N/A
Parvovirus Antibody Titre by HIA (Biobest)
(Canine parvovirus - HIA at Biobest)
Suitable Sample:
  • PC
Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 3ml whole blood
Turnaround Time:
  • Up to 4 weeks
Request/Test Included in:
  • Request test (must specify Biobest)
Comments:
  • Performed by haemagglutination inhibition
Internal Use Only:
Internal Codes: S11B
Internal Laboratory: Biobest
Internal Test Volume:
Parvovirus Antibody Titre by IFA
(Canine Parvovirus Antibody Titre by IFA)
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run Tuesday & Thursday(both CDV & CPV)
  • Cutoff time 7pm previuos working weekday
  • Results same day
Request/Test Included in:
  • CPV antibody titre by IFA
  • Combined CDV & CPV IFA titres
Comments:

Biobest now perform CPV titre testing by IFA (no longer HAI).

Canine Parvovirus (CPV) and Canine Distemper Virus (CDV) are serious viral pathogens in dogs that have been very well controlled with the use of vaccinations. Annual revaccination of dogs has been an industry norm that is being challenged due to increasing proof that protective immunity lasts for longer than one year (and often longer than 4 years) and the knowledge that vaccinations may in some cases have detrimental side effects ranging from anaphylaxis to immune-mediated disease.


Rather than merely stopping annual vaccination, dogs may have their immune status assessed to determine whether vaccination is indicated or not. This is particularly pertinent in at risk animals. Serological titre tests have been designed to measure the IgG levels and studies have shown that protection from infection can be determined with these tests.

INTERPRETATION:
  • Titres of less than 1:5 are considered seronegative and (re) vaccination is recommended.
  • Titres of >1:5 are considered seropositive.
  • Titres of >1:5 but <1:20 indicate that revaccination should be considered, especially if disease exposure risk is increased.
  • Dogs with titres of >1:20 probably do not require revaccination.
Internal Use Only:
  • Store at 2-8oC
  • Original file 21-07-2004
  • Serial dilutions are performed from 1:5 with four fold diltions to 1:8- (greater dilutions may be requested in some instances - charges are negotiable.
  • The use of EDTA or LH plasma has not been evaluated.
  • Samples can still be sent to Biobest in Scotland; this is done only if the client specifically requests it.
Internal Codes:
  • S11F (CPV by IFA)
  • S11L (CPV by IFA for a laboratory)
  • S14F (combinationCPV & CDV by IFA)
  • S14L (combinationCPV & CDV by IFA for laboratoried)
Internal Laboratory: VP
Internal Test Volume: 100-200 uL for both CPV and CDV
Parvovirus Antigen (Faeces)
Suitable Sample:
  • Faeces
  • Faecal swab
Unsuitable Sample:
  • Blood

 

Minimum Sample Size:
  • Faecal swab

 

Turnaround Time:
  • 3 hours

Request/Test Included in:
  • Request test

 

Comments:
  • False negative results may occur as the period of viral shedding in the faeces is relatively brief and virus is seldom detected 10 to 12 days after infection (this corresponds to 5 to 7 days of clinical illness).
  • False negatives may also occur very early in infection; retest in 36 to 48 hours if this is suspected.
  • Modified live vaccination results in transient faecal shedding and may give a weak positive result 5-15 days after vaccination.
Internal Use Only: AGEN Witness Parvo test in use
Internal Codes: S11A
Internal Laboratory: VP
Internal Test Volume:
PCV (Packed Cell Volume)
Suitable Sample:
  • EDTA
  • LH (but cannot do fibrinogen on LH)
Unsuitable Sample:
  • PC
  • Any other sample that has clotted
Minimum Sample Size:
  • 0.25 mL whole unclotted blood

 

Turnaround Time:
  • 2 hours as part of CBC

 

Request/Test Included in:
  • The PCV is part of the CBC and although it can be done as a single test, a full CBC is recommended
  • The fee for a repeat CBC is the same as a PCV (part CBC) and if the PCV is being done as a follow-up to a CBC done in the last 2 weeks, request a repeat CBC as this will provide much more information
Comments:
Internal Use Only: Recommend do CBC as very little extra cost and a lot more information.
Internal Codes:
  • HO1 for CBC
  • H02 for repeat CBC
  • H03 for part CBC
Internal Laboratory: VP
Internal Test Volume: <0.2 mL
Pepsinogen
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • LH
  • EDTA
Minimum Sample Size:
Turnaround Time:
  • 10 working days

 

Request/Test Included in:
  • Request test

 

Comments:
  • Confirm availability of test with lab before submitting sample

 

Internal Use Only:

Goes to Gribbles

Cost 13/6/2015 $51 + GST to us = $114.30 + GST to client.

Internal Codes:
Internal Laboratory: Gribbles
Internal Test Volume:
Peritoneal fluid analysis & cytology
(Fluid Analysis & Cytology (eg peritoneal, pleural fluids)
Suitable Sample:
  • Fresh smears made at the time of collection are useful.
  • EDTA is optimal for cytology.
  • Collect non-anticoagulated if sample is to be cultured; samples in EDTA are not suitable for culture.
Unsuitable Sample:
  • LH

 

Minimum Sample Size:
  • 0.5 mL if cell count is to be done
  • Smears for cytology only
Turnaround Time:
  • 24 hours Monday-Saturday
  • Most results are available later the same day
  • Samples submitted late in the day may be read the next morning (results available by 1 pm)
Request/Test Included in:
  • Request test

 

Comments:
  • If sufficient fluid, place some in an EDTA tube and the rest in either a PC tube (without activator) or a sterile urine container.

 

Internal Use Only:
Internal Codes:
  • C03
  • C03a (second site, same animal)
Internal Laboratory: VP
Internal Test Volume: 0.5 mL
Phenobarbitone
Suitable Sample:
  • PC
  • LH 
Unsuitable Sample:
  • EDTA
  • FL
Minimum Sample Size:
  • 2 ml whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Request test

 

Comments:
  • Collect sample at any time between doses.
  • More information on monitoring of therapeutic drugs is available on this website.
  • Thiopentone anaesthesia does not interfere.
Internal Use Only:
  • Client information sheet DCI-01 Drug Monitoring gives collection times for therapeuic monitoring of a range of drugs.
  • EDTA removed from suitable sample list 04-03-2009  MMcC
Internal Codes: B17
Internal Laboratory: VP
Internal Test Volume:
Phenobarbitone monitoring panel
(Monitoring panel - phenobarbitone)
Suitable Sample:
  • PC
Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
  • 2ml blood
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
Comments:
  • Includes phenobarbitone, ALT, AST, ALP, GGT and triglycerides
  • Collect sample at any time between doses
  • More information on monitoring of therapeutic drugs is available in client information sheets.
Internal Use Only:
Internal Codes: B37
Internal Laboratory:
Internal Test Volume:
Phosphorus (P)
Suitable Sample:
  • PC
  • LH
Unsuitable Sample:
  • EDTA
  • CIT
  • FL
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • CP 2, 6
  • FP 2, 5
  • EP 3
  • RP 2, 4
  • AP 1, 2, 3, 4
  • Request test
Comments:
  • Haemolysis will increase the value measured
  • Lipaemia will increase the value measured
Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 25 uL
Piroplasma Smears
Suitable Sample:
  • Air-dried blood smear
  • EDTA
Unsuitable Sample:
  • LH
  • PC
Minimum Sample Size:
Turnaround Time:
  • 24 hours Monday-Friday

 

Request/Test Included in:
  • Request test

 

Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume:
Piroplasmosis Serology
Suitable Sample:
  • PC

 

Unsuitable Sample:
  • EDTA
  • LH
Minimum Sample Size:
Turnaround Time:
  • 10 working days

 

Request/Test Included in:
  • Request test

 

Comments:
  • Contact Laboratory

 

Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: Dep't of Ag WA
Internal Test Volume:
Plant Identification
Suitable Sample:
  • Plant (pressed if not able to get to the laboratory in 24 hours)

 

Unsuitable Sample:
Minimum Sample Size:
Turnaround Time:
  • 10 working days

 

Request/Test Included in:
  • Request test

 

Comments:
  • Confirm availability of process with lab before submitting sample

 

Internal Use Only:
  • Where stock (including horses) are involved there is no charge to Vetpath
  • Need to charge courier out cost
Internal Codes: No code
Internal Laboratory:
  • WA Herbarium, Cnr George St & Hayman Rd, Kensington, WA 6152
  • Phone 9334 0500
  • Mark attention: R Davis
Internal Test Volume: n/a
Platelet Count - Automated
Suitable Sample:
  • EDTA 
  • CITRATE can be used, but note that there is a dilution effect of the sodium citrate on the platelet count.
Unsuitable Sample:
  • LH
  • PC
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • 3 hours (CBC), less if urgent

 

Request/Test Included in:
  • Request test

 

Comments:
  • Can be done as part of a CBC or separately
  • Results of automated count must correlate to what is seen on the smear
  • If does not correlate to the smear, a manual count may be recommended
  • Clumping of platelets or clots will decrease the count
Internal Use Only: {Notes for Internal Use}
Internal Codes: H05a
Internal Laboratory: VP
Internal Test Volume:
Platelet Count - Manual
Suitable Sample:
  • EDTA
  • CITRATE can be used, but note that there is a dilution effect of the sodium citrate on the platelet count.
Unsuitable Sample:
  • PC
  • LH
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • 3 hours, less if urgent

 

Request/Test Included in:
  • Coagulation screen
  • Individual test
Comments:
  • Results of manual count must correlate to what is seen on the smear
  • If does not correlate with the smear, this will be commented on
  • Clots will decrease platelet numbers for counting
Internal Use Only: {Notes for Internal Use}
Internal Codes:
  • H05
  • H16
Internal Laboratory: VP
Internal Test Volume:
Pleural fluid analysis & cytology
(Fluid analysis & cytology eg peritoneal, pleural fluids)
Suitable Sample:
  • Fresh smears made at the time of collection are useful.
  • EDTA is optimal for cytology
  • Collect non-anticoagulated if sample is to be cultured; samples in EDTA are not suitable for culture
Unsuitable Sample:
  • LH

 

Minimum Sample Size:
  • 0.5 mL if cell count is to be done
  • Smears for cytology only
Turnaround Time:
  • 24 hours Monday-Saturday
  • Most results are available later the same day
  • Samples submitted late in the day may be read the next morning (results available by 1 pm)
Request/Test Included in:
  • Request test

 

Comments:
  • If sufficient fluid, place some in an EDTA tube and the rest in either a PC tube (without activator) or a sterile urine container.

 

Internal Use Only:
Internal Codes:
  • C03
  • C03a (second site, same animal)
Internal Laboratory: VP
Internal Test Volume: 0.5 mL
PMSG
(Pregnant Mare Serum Gonadotrophin, equine chorionic gonadotrophin)
Suitable Sample:
  • PC preferred
  • LH plasma can be used
Unsuitable Sample:
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • Run once a week (Wednesday) 
  • Results available by Thursday am

 

Request/Test Included in:
  • Request test
  • All PMSGs are the ELISA test
  • The rapid RIM test is no longer available.
Comments:
  • Mare pregnancy 45 - 120 days
  • Optimum 70 days
Internal Use Only:
  • Use of the rapid RIM test was stopped 28-6-07 due to poor sensitivity
  • This has been replaced by the ELISA which is run once a week when there are samples to be done.
Internal Codes: E12
Internal Laboratory: VP
Internal Test Volume: 250 uL serum (two duplicates of 100 uL each)
Polyarthritis screen
Suitable Sample:
  • Joint fluid
  • Smears 
Unsuitable Sample:
  • Blood
Minimum Sample Size:
  • Two smears
Turnaround Time:
  • 24 hours
Request/Test Included in:
  • Request test
Comments:
  • The polyarthritis screen includes one full synovial fluid analysis and examination of up to 6 smears from other joints. 
  • The additional joints will receive a subjection description of cellularity and haemodilution, as well as a differential count.
Internal Use Only:
Internal Codes: C09
Internal Laboratory: VP
Internal Test Volume:
Polyoma Virus (Avian) by PCR
Suitable Sample:
  • LH blood
  • Feathers
  • Tissue (liver or spleen)
  • Droppings OR cloacal swab
Unsuitable Sample:
  • EDTA
  • PC
Minimum Sample Size:
  • Blood: volume of LH blood depends on size of bird, a few drops from smaller species is acceptable
  • Feathers: at least 3 freshly plucked feathers (send in small plastic bag or container)
Turnaround Time:
  • Approximately 2 weeks (confirm with laboratory)

 

Request/Test Included in:
  • Request test

 

Comments:
  • Also called budgerigar fledgling virus (but occurs in all psittacine birds)
  • PCR test
Internal Use Only:
  • Switched to Biobest Dec 2003 (no longer available via DNA solutions)
  • Test takes 1-2 days once sample received at Biobest
Internal Codes: G05
Internal Laboratory: Biobest, Scotland
Internal Test Volume:
Post-mortem Disposal
(no longer available)
Suitable Sample:
Unsuitable Sample: Live animal
Minimum Sample Size:
Turnaround Time:
Request/Test Included in: Request
Comments:
Internal Use Only: {Notes for Internal Use}
Internal Codes: T13-T19
Internal Laboratory: VP
Internal Test Volume:
Post-mortem Examination
(no longer available)
Suitable Sample: Dead animal
Unsuitable Sample: Live animals NOT accepted
Minimum Sample Size:
Turnaround Time: Monday to Friday, but always contact laboratory first
Request/Test Included in: Contact laboratory
Comments:
  • Post-mortems are not done at weekends unless by prior arrangement.
  • Always contact the laboratory prior to submission to confirm that the PM can be done.
  • Bodies are not returned.
  • Cremation can be arranged.
Internal Use Only: {Notes for Internal Use}
Internal Codes:
  • T08-T13
  • T16
Internal Laboratory: VP
Internal Test Volume:
Potassium (K)
Suitable Sample:
  • PC
  • LH
Unsuitable Sample:
  • EDTA
  • CIT
  • FL
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • 4 hours

 

Request/Test Included in:
  • Individual test
  • CP 2, 3, 4, 6
  • FP 2, 3, 5
  • EP 2, 3, 4, 5, 7
  • RP 2, 4
  • AP 2, 4
Comments:
  • LH decreases the problem of leakage of K from platelets during the clotting process and transit
  • Haemolysis will increase the value in some species
Internal Use Only: {Notes for Internal Use}
Internal Codes:
Internal Laboratory: VP
Internal Test Volume: 25 uL
Potassium bromide
(Bromide)
Suitable Sample:
  • PC
  • LH
Unsuitable Sample:
  • EDTA
  • CIT
  • FL
Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • 10 - 14 days 
Request/Test Included in:
  • Request test

 

Comments:
  • Collect sample 1 hour prior to the next dose of KBr
  • Mild lipaemia and/or haemolysis is acceptable.
  • More information on monitoring of therapeutic drugs is available on this website.
Internal Use Only:
  • Samples will be sent to Vetnostics Monday and Thursday.
  • Within run precision <3%
  • Between run precision >5%
  • All veterinary samples are run neat and diluted 1/5.
  • Client information sheet DCI-01 Drug Monitoring gives collection times for therapeuic monitoring of a range of drugs.
Internal Codes: B09
Internal Laboratory: Vetnostics (Mayne, Sydney) but actually run at RPA Hospital
Internal Test Volume:
  • 200 uL serum (at a pinch can get away with less)
  • Hemolysis does not interfere
Pre-anaesthetic screen
Suitable Sample:
  • EDTA
  • FL
  • PC
Unsuitable Sample:
Minimum Sample Size:
  • 1ml in each tube
Turnaround Time:
  • 4 hours
Request/Test Included in:
  • Request test
Comments:
  • Includes PCV (haematocrit), TS protein, ALT, ALP, creatinine, albumin, glucose.
Internal Use Only:
Internal Codes: PreAnS
Internal Laboratory: VP
Internal Test Volume:
Pregancy - Mare (>80 days)
(Oestrone Sulphate)
Suitable Sample:
  • PC
  • EDTA
Unsuitable Sample:
  • Haemolysed samples
  • Lipaemic samples
Minimum Sample Size:
  • 2 mL whole blood

 

Turnaround Time:
  • Test is done on Wednesdays (cutoff time 11:00 am)
  • Results are available by noon on Thursday at the latest
Request/Test Included in:
  • Request test

 

Comments:
  1. Samples should be separated and the serum/plasma stored in the freezer once it reaches Vetpath.
  2. Oestrone (estrone) sulphate is a steroid hormone detectable in the circulation in many species. It can be produced in the gonads, the adrenals and is produced by the foetal-placental unit during pregnancy.
  3. Pregnancy >80 days: Oestrone sulphate levels rise during pregnancy and are a good indicator of a viable pregnancy. In mares oestrone sulphate is detectable in the maternal circulation from early as 60 days post-mating until foaling. In the event of foetal death oestrone sulphate disappears rapidly from circulation.
  4. Cryptorchidism in horses 3 yo or older. See information sheet.
  5. Goat pregnancy >50 days post-service.
Internal Use Only:
  • Use the ELISA for all stallion/cryptorchid and non-equine samples
  • All pregnancy samples are to be done by ELISA on the following Wednesday unless:
  1. The sample is stamped "urgent".
  2. The pathologist requests the rapid test is to be used.
  3. If there is only one sample for the week and it is a pregnancy test, the scientist should ask Sue/Mary/John and it may be decided to use the rapid test.
Internal Codes:
  • E11E
  • E11EL (for laboratories)
Internal Laboratory: VP
Internal Test Volume:
Pregnancy - Bitch
(Relaxin)
Suitable Sample:
  • LH
  • PC 
Unsuitable Sample:
  • EDTA

 

Minimum Sample Size:
  • 1 mL whole blood

 

Turnaround Time:
  • 1 hour, Monday-Friday

 

Request/Test Included in:
  • Request test

 

Comments:
  • Relaxin is first detectable by week 4 of pregnancy, peaks at the 6-8th week then decreases.
  • Sample is stable at 2-8oC for < 48 hours, then freeze.
  • Haemolysis could obscure a weak positive result with the RIM methodology
Internal Use Only: RIM currently in use at Vetpath as the ELISA is not available, but check if necessary (Canine Pregancy Test, FASTest from Megacor )
    Internal Codes: E28
    Internal Laboratory: VP
    Internal Test Volume: 100 uL of serum or LH plasma
    Pregnancy - Camelid
    (Progesterone)
    Suitable Sample:
    • PC
    • Values in gel serum tubes may increase if stored in the tube
    Unsuitable Sample:
    • EDTA
    • LH
    Minimum Sample Size:
    • 2.5 mL whole blood

     

    Turnaround Time:
    • Monday-Friday with 2:00pm cutoff (results usually available by 1 pm)
    • Saturday by prior arrangement only
    Request/Test Included in:
    • Request test

     

    Comments:
    • Oestrus staging in bitches
    • Early pregnancy in mares
    • Pregnancy in camelids (collect samples 15-20 days after removal of male)
    • Pregnancy in sows
    Internal Use Only:
    Internal Codes: E14
    Internal Laboratory: VP
    Internal Test Volume: 150 uL
    Pregnancy - Mare (45 - 120 days)
    (PMSG / Pregnant Mare Serum Gonadotrophin, equine chorionic gonadotrophin)
    Suitable Sample:
    • PC preferred
    • LH plasma can be used
    Unsuitable Sample:
    Minimum Sample Size:
    • 1 mL whole blood

     

    Turnaround Time:
    • Run once a week (Wednesday)
    • Results available by Thursday am
    Request/Test Included in:
    • Request test
    • All PMSGs are the ELISA test
    • The rapid RIM test is no longer available.
    Comments:
    • Mare pregnancy 45 - 120 days
    • Optimum 70 days
    Internal Use Only:
    • Use of the rapid RIM test was stopped 28-6-07 due to poor sensitivity
    • This has been replaced by the ELISA which is run once a week when there are samples to be done.
    Internal Codes: E12
    Internal Laboratory: VP
    Internal Test Volume: 250 uL serum (two duplicates of 100 uL each)
    Premating Swab (bitch, dog, other)
    (See also Mare Swab)
    Suitable Sample:
    • Swab (vaginal, preputial)

     

    Unsuitable Sample:
    Minimum Sample Size:
    Turnaround Time:
    • 24 hours for culture
    • 48 hours for sensitivity with routine organisms
    Request/Test Included in:
    • Request test

     

    Comments:
    Internal Use Only: {Notes for Internal Use}
    Internal Codes: M05
    Internal Laboratory: VP
    Internal Test Volume:
    Profile (CBC and Biochemistry)
    Suitable Sample:
    • EDTA (for CBC/hematology/blood count)
    • PC (preferrred sample for biochemisty in mammals)
    • LH can be used for most, but not all, tests.
    • LH cannot be used for bile acids, GGT and GLDH
    • Fluoride anticoagulant is recommended for accurate measurement of glucose
    Unsuitable Sample:
    • CITRATE
    • See above for suitable samples
    Minimum Sample Size:
    • 1-2 mL of EDTA-anticoagulated whole blood for CBC
    • 2 mL of clotted
    Turnaround Time:
    • 4 hours minimum
    • Samples marked as "urgent" will be given priority, BUT may take longer to run as abnormal results may require further checking and dilutions.
    Request/Test Included in:
    • Specific profile (species and level)
    • If the sample is below the optimal volume, please prioritise the tests you consider most important
    Comments:  
    Internal Use Only:
    Internal Codes:
    Internal Laboratory: VP
    Internal Test Volume:
    Progesterone
    Suitable Sample:
    • PC
    • Values in gel serum tubes may increase if stored in the tube
    Unsuitable Sample:
    • EDTA
    • LH
    Minimum Sample Size:
    • 2.5 mL whole blood

     

    Turnaround Time:
    • Monday-Friday with 2:00pm cutoff (results usually available by 1 pm) 
    • Saturday by prior arrangement only
    Request/Test Included in:
    • Request test

     

    Comments:
    • Oestrus staging in bitches
    • Early pregnancy in mares
    • Pregnancy in camelids (collect samples 15-20 days after removal of male) 
    • Pregnancy in sows
    Internal Use Only: {Notes for Internal Use}
    Internal Codes: E14
    Internal Laboratory: VP
    Internal Test Volume: 150 uL
    Prolactin
    Suitable Sample:
    • PC

     

    Unsuitable Sample:
    • LH
    • EDTA
    Minimum Sample Size:
    • 1 mL whole blood

     

    Turnaround Time:
    • Minimum of 5-7 working days
    • Contact VETPATH as this sample is sent to an external referral laboratory 
    Request/Test Included in:
    • Request test

     

    Comments:
    Internal Use Only: Price of $60.65 at time of record update to Gribbles 25-7-2009
    Internal Codes: E29
    Internal Laboratory: Gribbles
    Internal Test Volume: 200 uL ?? Need to check this
    Prostatic Fluid Cytology
    Suitable Sample:
    • EDTA is optimal for cytology
    • PC/H if sample is to be cultured
    Unsuitable Sample:
    • LH

     

    Minimum Sample Size:
    • 0.5 mL

     

    Turnaround Time:
    • 24 hours Monday-Saturday
    • Most results are available later the same day
    • Samples submitted late in the day may be read the next morning (results available by 1 pm)
    Request/Test Included in:
    • Request test

     

    Comments:
    • If sufficient fluid, place some in an EDTA tube and the rest in either a PC tube (without activator) or a sterile urine container.

    Internal Use Only: {Notes for Internal Use}
    Internal Codes: C06
    Internal Laboratory: VP
    Internal Test Volume:
    Protein (total protein)
    (Total Protein)
    Suitable Sample:
    • PC - serum protein
    • LH - plasma protein
    • EDTA - plasma protein
    Unsuitable Sample:
    • Fluoride oxalate (glucose tube)

     

    Minimum Sample Size:
    • 2 mL whole blood

     

    Turnaround Time:
    • 3 hours

     

    Request/Test Included in:
    • CP1, 2, 3, 4, 5, 6
    • FP1, 2, 3, 4, 5
    • EP1, 2, 3
    • RP1, 2, 3, 4
    • AP1, 2, 3, 4
    • Request test
    Comments:
    Internal Use Only: Have used fluoride EDTA previously but there is no information with the current reagents to indicate that this is or is not suitable.
    Internal Codes:
    Internal Laboratory: VP
    Internal Test Volume: 25 uL
    Prothrombin Time
    (PT)
    Suitable Sample:
    • CIT

     

    Unsuitable Sample:
    • PC
    • LH
    • EDTA
    Minimum Sample Size:
    • Filled CIT tube for PT only
      PLUS
    • 2 mL EDTA if doing coagulation screen (including CBC)
    Turnaround Time:
    • 2 hours (less if urgent)

     

    Request/Test Included in:
    • Request PT
    • Request PT/PTT
    • Coagulation screen

    Comments:
    • Beware of insufficiently filled OR overfilled CIT tubes; tubes must be filled to the line marked on the tube.
    • Beware of clots in the sample.
    • PT is the most sensitive indicator of vitamin K-rodenticide toxicity
    • If using the PT to monitor adequacy of duration of treatment, measure the PT 48 hours after discontinuation of vitamin K therapy
    Internal Use Only: {Notes for Internal Use}
    Internal Codes:
    • H11
    • H12
    • H15
    Internal Laboratory: VP
    Internal Test Volume:
    Psittacine Beak & Feather Disease
    Suitable Sample:
    • At least 3 freshly plucked feathers.  These should be sealed in a small plastic bag or container.
      and/or
    • LH blood: the volume should reflect the size of the bird, only a few drops are needed from small birds.
    Unsuitable Sample:
    • EDTA
    • PC
    Minimum Sample Size:
    • Blood: volume of LH blood depends on size of bird, a few drops from smaller species is acceptable
    • Feathers: at least 3 freshly plucked feathers (send in small plastic bag or container).
    Turnaround Time:
    • Approximately 2 weeks (confirm with laboratory)

     

    Request/Test Included in:
    • Request test

     

    Comments:
    • PCR test for Circovirus

     

    Internal Use Only:
    • Switched to Biobest Dec 2003 (no longer available via Gary Cross at Sydney University)
    • Testing takes 1-2 days once sample received.
    Internal Codes: G04
    Internal Laboratory: Biobest, Scotland
    Internal Test Volume:
    Psittacosis (avian)
    (Chlamydia)
    Suitable Sample:
    • 2 air-dried smears of exudate (area the size of a 5 cent coin on each slide)
    • Feline samples: conjunctival or exudate
    • Avian samples: cloacal swab/smear, fresh liver
    • Do NOT refrigerate
    Unsuitable Sample:
    • Samples in transport media.

     

    Minimum Sample Size:
    • 1 smear

     

    Turnaround Time:
    • Monday - Friday on demand
    • 3:00 pm cutoff
    • Results by 6 pm
    Request/Test Included in:
    • Request test

     

    Comments:
    • Do not refrigerate smears.

     

    Internal Use Only:
    Internal Codes:
    • P02
    • P03
    Internal Laboratory: VP
    Internal Test Volume:
    PT
    (Prothrombin time)
    Suitable Sample:
    • CIT
    Unsuitable Sample:
    • EDTA
    • PC
    • FL
    • LH
    Minimum Sample Size:
    • Fill tube to correct level
    Turnaround Time:
    • 1 hour
    Request/Test Included in:
    • Request PT
    • PT/PTT
    • Coagulation profile
    Comments:
    • Beware of insufficiently filled OR overfilled CIT tubes; tubes must be filled to the line marked on the tube.
    • Beware of clots in the sample.
    • PT is the most sensitive indicator of vitamin K-rodenticide toxicity
    • If using the PT to monitor adequacy of duration of treatment, measure the PT 48 hours after discontinuation of vitamin K therapy
    Internal Use Only:
    Internal Codes:
    Internal Laboratory:
    Internal Test Volume:
    PTH
    (Parathyroid Hormone)
    Suitable Sample:
    • PC
    Unsuitable Sample:
    • EDTA
    • LH
    • SC
    Minimum Sample Size:
    • At least 2.0 mL whole blood
    • Submit an extra sample for serum calcium concentration

     

    Turnaround Time:
    • At least 10 -14 working weekdays
    Request/Test Included in:
    • Request Test, contact laboratory in advance.

     

    Comments:
    • Sample must be centrifuged within 1 - 2 hours of collection.
    • Contact the laboratory to discuss with a pathologist before submission of the sample.
    Internal Use Only:

    Send to Michigan state university.

    Internal Codes: E36R
    Internal Laboratory: MSU
    Internal Test Volume: 1ml serum
    PTH-related Protein (Parathyroid Hormone-related Protein)
    Suitable Sample:
    • EDTA

    Unsuitable Sample:
    • PC
    • LH
    Minimum Sample Size:
    • 2.0 ml whole blood
    Turnaround Time:
    • 10 - 14 working days

     

    Request/Test Included in:
    • Request Test, contact laboratory for current price.

     

    Comments:
      • Contact the laboratory to discuss with a pathologist before submission of the sample.
    • Samples must be centrifuged within the first hour.
    • Sample should not be lipaemic (fasted).
    • Plasma must be sent frozen.


    Internal Use Only:
    • Send to Michigan state University
    Internal Codes: E36aR
    Internal Laboratory: MSU
    Internal Test Volume: Minimum 500 uL plasma
    PTT
    (Partial Prothrombin Time)
    Suitable Sample:
    • CIT

     

    Unsuitable Sample:
    • PC
    • LH
    • EDTA
    Minimum Sample Size:
    • Filled CIT tube for PT only
      PLUS
    • 2 mL EDTA if doing coagulation profile (including CBC)
    Turnaround Time:
    • 2 hours (less if urgent)

     

    Request/Test Included in:
    • Request PT
    • Request PT/PTT
    • Coagulation screen
    Comments:
    • Beware of insufficiently filled OR overfilled CIT tubes; tubes must be filled to the line marked on the tube.
    • Beware of clots in the sample.
    Internal Use Only:
    Internal Codes:
    • H11
    • H12
    • H15
    Internal Laboratory: VP
    Internal Test Volume: